Species differences in impairment and recovery of alveolar macrophage functions following single and repeated ozone exposures

Journal Article · Thu Aug 01 00:00:00 EDT 1991 · Toxicology and Applied Pharmacology; (United States)

DOI:https://doi.org/10.1016/0041-008X(91)90299-T· OSTI ID:5226774

Oosting, R S; van Golde, L M; Verhoef, J; Van Bree, L ^[1]

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1. Laboratory for Toxicology, National Institute of Public Health and Environmental Protection, Bilthoven, (Netherlands)

Effects of single (0.4 ppm for 3, 6, or 12 hr) and repeated (0.4 ppm, 12 hr/day for 3 or 7 days) in vivo ozone exposures on rat and mouse alveolar macrophage functions and cell number were investigated. Single ozone exposure of rats resulted in a small (approximately 15%) decrease in Fc-receptor-mediated phagocytosis and phorbol ester-induced superoxide production by the alveolar macrophages and was followed by recovery above control levels within 12 hr of exposure. Repeated exposures of rats for up to 7 days did not alter alveolar macrophage functions, with the exception of the effects of 3 days of exposure on superoxide production (71 {plus minus} 9% as compared with the controls). In mice, significant changes in alveolar macrophage functions were not observed until 12 hr of exposure (at that timepoint phagocytosis was 74 {plus minus} 2%). Repeated ozone exposures of mice did not cause a further decrease in phagocytosis (at Day 7, 74 {plus minus} 14%). Both after 3 and 7 days of repeated ozone exposure of mice, superoxide production by the alveolar macrophages was inhibited approximately 50%. In rats and mice, repeated ozone exposures led to an increase in the number of alveolar macrophages. In mice, this increase appeared at a later time point (at Day 7 vs Day 3) and was less pronounced (at Day 7, 139 {plus minus} 9% vs 179 {plus minus} 17%) as compared with rats. In summary, our data show that rat and mouse alveolar macrophages have different susceptibilities to both single and repeated in vivo ozone exposures.

• OSTI ID: 5226774
• Journal Information: Toxicology and Applied Pharmacology; (United States), Vol. 110:1; ISSN 0041-008X
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
MACROPHAGES
BIOLOGICAL RECOVERY
OZONE
TOXICITY
ACUTE EXPOSURE
CHRONIC EXPOSURE
GENETIC VARIABILITY
INHALATION
LAVAGE
MICE
NEUTROPHILS
PHAGOCYTOSIS
RATS
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BIOLOGICAL VARIABILITY
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
INTAKE
LEUKOCYTES
MAMMALS
MATERIALS
PHAGOCYTES
RECOVERY
RODENTS
SOMATIC CELLS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology