NMR studies on the structure and dynamics of lac operator DNA
Nuclear Magnetic Resonance spectroscopy was used to elucidate the relationships between structure, dynamics and function of the gene regulatory sequence corresponding to the lactose operon operator of Escherichia coli. The length of the DNA fragments examined varied from 13 to 36 base pair, containing all or part of the operator sequence. These DNA fragments are either derived genetically or synthesized chemically. Resonances of the imino protons were assigned by one dimensional inter-base pair nuclear Overhauser enhancement (NOE) measurements. Imino proton exchange rates were measured by saturation recovery methods. Results from the kinetic measurements show an interesting dynamic heterogeneity with a maximum opening rate centered about a GTG/CAC sequence which correlates with the biological function of the operator DNA. This particular three base pair sequence occurs frequently and often symmetrically in prokaryotic nd eukaryotic DNA sites where one anticipates specific protein interaction for gene regulation. The observed sequence dependent imino proton exchange rate may be a reflection of variation of the local structure of regulatory DNA. The results also indicate that the observed imino proton exchange rates are length dependent.
- Research Organization:
- Pennsylvania Univ., Philadelphia (USA)
- OSTI ID:
- 5221047
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
DNA
NUCLEAR MAGNETIC RESONANCE
STRUCTURAL CHEMICAL ANALYSIS
BIOCHEMICAL REACTION KINETICS
EXPERIMENTAL DATA
GENE REGULATION
OVERHAUSER EFFECT
PROTONS
BARYONS
DATA
ELEMENTARY PARTICLES
FERMIONS
HADRONS
INFORMATION
KINETICS
MAGNETIC RESONANCE
NUCLEIC ACIDS
NUCLEONS
NUMERICAL DATA
ORGANIC COMPOUNDS
REACTION KINETICS
RESONANCE
550200* - Biochemistry
400101 - Activation
Nuclear Reaction
Radiometric & Radiochemical Procedures