Endogenous biosynthesis of nitrate and its role in vivo nitrosation. [Mustela putorius furo]

Thesis/Dissertation · Tue Jan 01 00:00:00 EST 1985

OSTI ID:5219925

Dull, B J

The ferret (Mustela putorius furo) was chosen as a model animal for the metabolism of nitrate. The net biosynthesis of nitrate in the ferret was demonstrated to be 8.89-10.3 mcmole/kg/day. Nitrate balance studied indicate that, because of metabolism, excretion of nitrate was lower than ingestion which oral doses were higher than 6.3 mcmole/day. At nitrate ingestions levels of less than 6.3 mcmole/day, excretion exceeded intake. Studies with 15-N labelled nitrate indicated that only 36% of the oral dose was recovered as 15-N nitrate from urine. Oral doses of 15-N labelled ammonia resulted in the incorporation of the 15-N label into nitrate in urine and feces. This demonstrated that ammonia can serve as a precursor to biosynthesized nitrate. Oral dosing of ferrets with N-nitrosoproline results in 94.7% recovery in the urine within 48 hr. 15-N labelled N-nitrosoproline could not be detected in the urine at anytime during a ten day feeding study with 15-N labelled nitrate. 14-N labelled N-nitrosoproline was detected throughout. As 14-N N-nitrosoproline could not be detected in the diet, it would appear that excretion of 14-N labelled N-nitrosoproline must be arising from an in vivo nitrosation site other than the stomach.

• Research Organization: Cornell Univ., Ithaca, NY (USA)
• OSTI ID: 5219925
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
NITRATES
BIOSYNTHESIS
EXCRETION
METABOLISM
AMMONIA
EXPERIMENTAL DATA
INGESTION
LABELLED COMPOUNDS
MAMMALS
NITROGEN 14
NITROGEN 15
NITROSO COMPOUNDS
PRECURSOR
PROLINE
TRACER TECHNIQUES
URINE
AMINES
AMINO ACIDS
ANIMALS
AZOLES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
CARBOXYLIC ACIDS
CLEARANCE
DATA
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDRIDES
HYDROGEN COMPOUNDS
INFORMATION
INTAKE
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MATERIALS
NITROGEN COMPOUNDS
NITROGEN HYDRIDES
NITROGEN ISOTOPES
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PYRROLES
PYRROLIDINES
STABLE ISOTOPES
SYNTHESIS
VERTEBRATES
WASTES
550201* - Biochemistry- Tracer Techniques