Inactivation of the Hutchinson strain of non-A, non-B hepatitis virus by combined use of beta-propiolactone and ultraviolet irradiation

Journal Article · Sat Jun 01 00:00:00 EDT 1985 · J. Med. Virol.; (United States)

DOI:https://doi.org/10.1002/jmv.1890160204· OSTI ID:5217657

Prince, A M; Stephan, W; Dichtelmueller, H B; Brotman, B; Huima, T

A beta-propiolactone/ultraviolet irradiation procedure (beta PL/UV) has been evaluated for its ability to inactivate 30,000 chimpanzee infectious doses of the Hutchinson strain of non-A, non-B (NANB) virus. The chimpanzees were inoculated with plasma to which this dose of the titrated virus had been added prior to application of the beta PL/UV process in accordance with a procedure used for licensed blood derivatives in Germany. Neither animal developed hepatitis. When subsequently challenged with the same contaminated plasma, which had not been sterilized, both animals promptly developed typical NANB hepatitis. This study extends the high (approximately 10(7)-fold) process efficiency of the beta PL/UV procedure previously reported for hepatitis B virus to a blood-borne NANB virus.

• Research Organization: Lindsley F. Kimball Research Institute of the New York Blood Center, NY
• OSTI ID: 5217657
• Journal Information: J. Med. Virol.; (United States), Vol. 2
• Country of Publication: United States
• Language: English

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