Altered expression of the cyclin D1 and retinoblastoma genes in human esophageal cancer
- Columbia Univ., New York, NY (United States)
- International Agency for Research on Cancer, Cedex (France)
- National Cancer Institute, Bethesda, MD (United States)
- Chinese Academy of Medical Sciences, Beijing (China)
The authors have examined DNA from four human esophageal carcinoma cell lines and 50 primary esophageal carcinomas obtained from China, Italy, and France for amplification of the cyclin D1 gene. They also examined 36 of these 50 carcinomas for expressions of the cyclin D1 and retinoblastoma (RB) proteins by immunohistochemistry. They found a 3- to 10-fold amplification of the cyclin D1 gene in 16 of the 50 (32%) tumors and in two of the four cell lines. Cyclin D1 protein was overexpressed in 12 of 13 tumors and the two cell lines that showed gene amplification when compared to normal controls. Studies on RB protein expression indicated that 6 of the 36 (17%) tumor samples examined and one cell line did not show detectable expression of this protein. The tumors and cell lines that had cyclin D1 gene amplification and overexpression exhibited normal levels of expression of RB protein. By contrast, the tumors and cell line that did not appear to express the RB protein did not show amplification of the cyclin D1 gene and expressed only low levels of the cyclin D1 protein (P = 0.03). These results suggest that the inhibitory effect of RB on cell cycle progression can be abrogated during tumor development either by loss of expression of the RB gene or by increased expression of the cyclin D1 gene. 46 refs., 5 figs., 2 tabs.
- OSTI ID:
- 5216062
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 90:19; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
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