skip to main content

Title: Evaluation of nitrate reductase activity in Rhizobium japonicum

Nitrate reductase activity was evaluated by four approaches, using four strains of Rhizobium japonicum and 11 chlorate-resistant mutants of the four strains. It was concluded that in vitro assays with bacteria or bacteroids provide the most simple and reliable assessment of the presence or absence of nitrate reductase. Nitrite reductase activity with methyl viologen and dithionite was found, but the enzyme activity does not confound the assay of nitrate reductase. 18 references
Authors:
;
Publication Date:
OSTI Identifier:
5216055
Resource Type:
Journal Article
Resource Relation:
Journal Name: Appl. Environ. Microbiol.; (United States); Journal Volume: 46:2
Research Org:
Dept. of Agronomy, Ohio State Univ. and Ohio Agricultural Research and Development Center, Wooster, OH 44691
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; MYCORRHIZAS; ENZYME ACTIVITY; NITROGEN FIXATION; IN VITRO; INHIBITION; MUTANTS; NITRATES; OXIDOREDUCTASES; SYMBIOSIS; ENZYMES; NITROGEN COMPOUNDS; OXYGEN COMPOUNDS 550700* -- Microbiology