Regulation of metallothionein gene expression and cellular zinc accumulation in a rat small intestinal cell line

Conference · Fri Mar 15 00:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5215123

Carlson, J M; Cousins, R J ^[1]

---

1. Univ. of Florida, Gainesville (United States)

The effects of extracellular zinc concentration on metallothionein gene expression and cellular zinc accumulation were studied in IRD-98 cells. This is a non-transformed clonal line established by Negrel, et al. from fetal rat small intestine which possess characteristics of small bowel epithelial cells. Cells were maintained in DMEM and grown to confluent monolayers. The response to media zinc concentrations over the range of 5-150 {mu}mol/L was assessed. After 24 h in culture, cell zinc and metallothionein protein concentrations were significantly increased in cells provided higher levels of media zinc. Subsequent time course experiments showed that cells exposed to higher zinc levels had significant elevations in both metallothionein mRNA, peaking at 24 h, and metallothionein protein increasing through 48 h. Furthermore, cell zinc concentrations were significantly increased. At 48 h of culture, greater than 50% of the additional cellular zinc accumulated could be attributed to elevated metallothionein protein levels. These cells represent a zinc-responsive model to examine the mechanism of zinc uptake and transcellular transport by intestinal cells and the regulatory factors involved.

• OSTI ID: 5215123
• Report Number(s): CONF-9104107-; CODEN: FAJOE
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 5:5; Conference: 75. annual meeting of the Federation of American Societies for Experimental Biology (FASEB), Atlanta, GA (United States), 21-25 Apr 1991; ISSN 0892-6638
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
METALLOTHIONEIN
GENES
ZINC
BIOLOGICAL EFFECTS
BIOLOGICAL ACCUMULATION
CLONE CELLS
EPITHELIUM
GENE REGULATION
MEMBRANE TRANSPORT
METABOLISM
SMALL INTESTINE
TISSUE DISTRIBUTION
ANIMAL TISSUES
BODY
CELL CULTURES
DIGESTIVE SYSTEM
DISTRIBUTION
ELEMENTS
GASTROINTESTINAL TRACT
INTESTINES
METALLOPROTEINS
METALS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
TISSUES
560300* - Chemicals Metabolism & Toxicology