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Title: Cell survival kinetics in peripheral blood and bone marrow during total body irradiation for marrow transplantation

Cell survival kinetics in both peripheral blood and in bone marrow have been studied over the time course of hyperfractionated total body irradiation (TBI) for bone marrow transplantation. Our unique TBI regimen allows the study of the in vivo radiation effect uncomplicated by prior cyclophosphamide, since this agent is given after TBI in our cytoreduction scheme. Peripheral blood cell concentrations were monitored with conventional laboratory cell counts and differentials. Absolute bone marrow cell concentrations were monitored by measuring cell concentrations in an aspirate sample and correcting for dilution with blood by a cell cycle kinetic method using cytofluorometry. For lymphocytes in peripheral blood in patients in remission, the effective D/sub 0/ ranged from 373 rad in 10 children less than or equal to 10 y old, to 536 rad in the four patients between 11 to 17 y old, while n = 1.0 in all groups. There was no trend observed according to age. Granulocytes had a much higher effective D/sub 0/, approximately 1000 rad in vivo. Absolute nucleated cell concentration in marrow dropped slowly initially, due to an increased lymphocyte concentration in marrow during a concurrent drop in lymphocyte concentration in peripheral blood, but eventually fell on the lastmore » day of TBI ranging from 7 to 44% of the initial marrow nucleated cell concentration. Marrow myeloid elements, however, dropped continuously throughout the course of TBI.« less
Authors:
; ;
Publication Date:
OSTI Identifier:
5211575
Resource Type:
Journal Article
Resource Relation:
Journal Name: Int. J. Radiat. Oncol., Biol. Phys.; (United States); Journal Volume: 9:11
Research Org:
Memorial Sloan-Kettering Cancer Center, New York, NY
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 62 RADIOLOGY AND NUCLEAR MEDICINE; BLOOD CELLS; BIOLOGICAL RADIATION EFFECTS; POPULATION DYNAMICS; BONE MARROW CELLS; RADIOTHERAPY; SIDE EFFECTS; BLOOD COUNT; CELL FLOW SYSTEMS; FRACTIONATED IRRADIATION; HARD X RADIATION; LEUKEMIA; LYMPHOCYTES; PATIENTS; TRANSPLANTS; WHOLE-BODY IRRADIATION; ANIMAL CELLS; BIOLOGICAL EFFECTS; BIOLOGICAL MATERIALS; BLOOD; BODY FLUIDS; CONNECTIVE TISSUE CELLS; DISEASES; ELECTROMAGNETIC RADIATION; EXTERNAL IRRADIATION; HEMIC DISEASES; IONIZING RADIATIONS; IRRADIATION; LEUKOCYTES; MATERIALS; MEDICINE; NEOPLASMS; NUCLEAR MEDICINE; RADIATION EFFECTS; RADIATIONS; RADIOLOGY; SOMATIC CELLS; THERAPY; X RADIATION 560151* -- Radiation Effects on Animals-- Man; 560121 -- Radiation Effects on Cells-- External Source-- (-1987); 550603 -- Medicine-- External Radiation in Therapy-- (1980-)