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Title: Some physiological aspects of nitrate reductase-deficient Nicotiana plumbaginifolia plants

Abstract

Chlorate-resistant Nicotiana plumbaginifolia (cv. Viviani) mutants were found to be defective in the nitrate reductase apoprotein (NR/sup -/ nia). Because they could not grow with nitrate as sole nitrogen source, they were cultivated as graftings on wild type Nicotiana tabacum. The grafts of NR/sup -/ plants were found to contain less malate but more amino acids, sugars and starch than the wild type. Moreover they were chlorotic, with a slight increase of the proportion of LH Chl a/b protein complexes and they exhibited a lowering of the efficiency of energy transfer between the light-harvesting complexes and the active centers. After /sup 14/CO/sub 2/ pulse and chase experiments. The total /sup 14/C incorporation of the mutant leaves was approximately 20% of that of the control. The NR/sup -/ leaves mainly accumulated /sup 14/C in the whole intermediates of the Calvin-cycle and in sucrose. In the most deficient NR leaves, chloroplasts were stuffed with large starch inclusions disorganizing the lamellar system.

Authors:
; ; ;
Publication Date:
Research Org.:
I.N.R.A., Versailles, France
OSTI Identifier:
5207435
Resource Type:
Conference
Journal Name:
Plant Physiol., Suppl.; (United States)
Additional Journal Information:
Journal Volume: 80:4; Conference: Annual meeting of the American Society of Plant Physiologists, Baton Rouge, LA, USA, 8-12 Jun 1986
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CARBON 14; BIOLOGICAL ACCUMULATION; NICOTIANA; ENZYMES; MUTANTS; OXIDOREDUCTASES; PHYSIOLOGY; NITRATES; REDUCTION; AMINO ACIDS; BIOCHEMISTRY; CHLORATES; LEAVES; RADIOISOTOPES; SACCHARIDES; STARCH; TOBACCO; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CARBOHYDRATES; CARBON ISOTOPES; CARBOXYLIC ACIDS; CHEMICAL REACTIONS; CHEMISTRY; CHLORINE COMPOUNDS; EVEN-EVEN NUCLEI; HALOGEN COMPOUNDS; ISOTOPES; LIGHT NUCLEI; NITROGEN COMPOUNDS; NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; OXYGEN COMPOUNDS; PLANTS; POLYSACCHARIDES; REAGENTS; YEARS LIVING RADIOISOTOPES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Saux, C, Morot-Gaudry, J F, Lemoine, Y, and Caboche, M. Some physiological aspects of nitrate reductase-deficient Nicotiana plumbaginifolia plants. United States: N. p., 1986. Web.
Saux, C, Morot-Gaudry, J F, Lemoine, Y, & Caboche, M. Some physiological aspects of nitrate reductase-deficient Nicotiana plumbaginifolia plants. United States.
Saux, C, Morot-Gaudry, J F, Lemoine, Y, and Caboche, M. 1986. "Some physiological aspects of nitrate reductase-deficient Nicotiana plumbaginifolia plants". United States.
@article{osti_5207435,
title = {Some physiological aspects of nitrate reductase-deficient Nicotiana plumbaginifolia plants},
author = {Saux, C and Morot-Gaudry, J F and Lemoine, Y and Caboche, M},
abstractNote = {Chlorate-resistant Nicotiana plumbaginifolia (cv. Viviani) mutants were found to be defective in the nitrate reductase apoprotein (NR/sup -/ nia). Because they could not grow with nitrate as sole nitrogen source, they were cultivated as graftings on wild type Nicotiana tabacum. The grafts of NR/sup -/ plants were found to contain less malate but more amino acids, sugars and starch than the wild type. Moreover they were chlorotic, with a slight increase of the proportion of LH Chl a/b protein complexes and they exhibited a lowering of the efficiency of energy transfer between the light-harvesting complexes and the active centers. After /sup 14/CO/sub 2/ pulse and chase experiments. The total /sup 14/C incorporation of the mutant leaves was approximately 20% of that of the control. The NR/sup -/ leaves mainly accumulated /sup 14/C in the whole intermediates of the Calvin-cycle and in sucrose. In the most deficient NR leaves, chloroplasts were stuffed with large starch inclusions disorganizing the lamellar system.},
doi = {},
url = {https://www.osti.gov/biblio/5207435}, journal = {Plant Physiol., Suppl.; (United States)},
number = ,
volume = 80:4,
place = {United States},
year = {Tue Apr 01 00:00:00 EST 1986},
month = {Tue Apr 01 00:00:00 EST 1986}
}

Conference:
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