Effect of ozone on photosynthesis in Euglena gracilis Z

Conference · Tue Apr 01 00:00:00 EST 1986 · Plant Physiol., Suppl.; (United States)

OSTI ID:5202169

Chevrier, N; Sarhan, F; Chung, Y S

Euglena cells were used as a plant cell model to study the effects of O/sub 3/ on photosynthesis. Cells were exposed to 1 ..mu..mole min/sup -1/ O/sub 3/ and viability, as estimated by the trypan blue exclusion test, was 80% after a 2 hr exposure period. In vivo /sup 14/CO/sub 2/ fixation was inhibited by 77% after the 2 hr O/sub 3/ exposure as compared to control (air treated). On the other hand, the Hill reaction rate (DCPI reduction) and chlorophyll content were not significantly changed. These results suggest that O/sub 3/ penetrate beyond the chloroplast envelope and affect the content of stroma, possibly by oxidizing essential SH groups of the CO/sub 2/ reduction cycle enzymes without affecting the thylakoid membranes function. After O/sub 3/ exposure, Euglena cells were transferred to a growth chamber under autotrophic conditions and allowed to recuperate. /sup 14/CO/sub 2/ fixation capacity was totally restored after 20 hrs, indicating that the cells were able to repair the damage inflicted to chloroplasts during O/sub 3/ exposure.

• Research Organization: Univ. of Quebec, Montreal
• OSTI ID: 5202169
• Journal Information: Plant Physiol., Suppl.; (United States), Vol. 80:4; Conference: Annual meeting of the American Society of Plant Physiologists, Baton Rouge, LA, USA, 8-12 Jun 1986
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
EUGLENA
INJURIES
PHOTOSYNTHESIS
OZONE
BIOLOGICAL EFFECTS
CARBON 14 COMPOUNDS
CARBON DIOXIDE FIXATION
TRACER TECHNIQUES
ALGAE
CHEMICAL REACTIONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MASTIGOPHORA
MICROORGANISMS
PHOTOCHEMICAL REACTIONS
PLANTS
SYNTHESIS
UNICELLULAR ALGAE
560303* - Chemicals Metabolism & Toxicology- Plants- (-1987)