Molecular biology of coal bio-desulfurization. [Rhodococcus rhodochrous, Desulfovibrio desulfuricans]

Technical Report · Thu Apr 30 00:00:00 EDT 1992

OSTI ID:5195928

Young, K D; Gallagher, J R

Genes cloned from Rhodococcus rhodochrous IGTS8 can transfer the DBT desulfurization phenotype to a different species (R. Fascians). The product was identified as 2-phenylphenol by gas chromatography. This result parallels the results we have previously reported for the activity of these genes in a DBT-negative mutant of IGTS8. Thus, the evidence is strong that we have identified and cloned the entire set of genes that are responsible for this very specific desulfurization reaction. Sequencing of these genes has commenced. A genomic library was constructed from the bacterium, Besulfovibrio desulfuricans. Screening has not yet identified a clone that carries the desulfurization genes from that organism. Two open reading frames, doxH and doxJ, in the C18 DBT degradation pathway were mutated and are now believed to be dispensable to that pathway. Finally, progress was made toward beginning to sequence the DBT dixoygenase genes from strain A15.

• Research Organization: USDOE Pittsburgh Energy Technology Center, PA (United States)
• Sponsoring Organization: USDOE; USDOE, Washington, DC (United States)
• DOE Contract Number: AC22-89PC89901
• OSTI ID: 5195928
• Report Number(s): DOE/PC/89901-T11; ON: DE92015499
• Country of Publication: United States
• Language: English

Related Subjects

01 COAL, LIGNITE, AND PEAT
59 BASIC BIOLOGICAL SCIENCES
COAL
DESULFURIZATION
GENES
CLONING
MICROORGANISMS
SULFUR COMPOUNDS
BIODEGRADATION
CHEMICAL REACTION KINETICS
ENZYMES
GAS CHROMATOGRAPHY
PROGRESS REPORT
CARBONACEOUS MATERIALS
CHEMICAL REACTIONS
CHROMATOGRAPHY
DECOMPOSITION
DOCUMENT TYPES
ENERGY SOURCES
FOSSIL FUELS
FUELS
KINETICS
MATERIALS
ORGANIC COMPOUNDS
PROTEINS
REACTION KINETICS
SEPARATION PROCESSES
010402* - Coal
Lignite
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550700 - Microbiology
550400 - Genetics