Solvent extraction of low-molecular-weight polycyclic aromatic hydrocarbons from reversed-phase liquid chromatographic effluents
High-performance liquid chromatography provides an effective means of separating constituents of samples of environmental origin. When such samples contain large numbers of constituents at low concentrations, retention times and detector responses provide insufficient data for component characterization. Coupling of HPLC fractionation with GC/MS characterization has proven to be a powerful technique for determining compounds of interest. Reversed-phase HPLC procedures are the most effective for separation of samples containing low-molecular-weight polycyclic aromatic hydrocarbons. However, the aqueous methanol or acetonitrile solvents used with reversed-phase HPLC are incompatible with high-resolution, high-sensitivity gas chromatography. Reversed-phase solvents can be removed from a sample by distillation or by evaporation with an inert gas. Both of these techniques also lead to significant losses of volatile analytes such as low-molecular-weight polycyclic aromatic hydrocarbons. Solvent extraction with large volumes of pentane has been used to isolate polycyclic aromatic hydrocarbons from HPLC effluents containing methanol and from low-pressure liquid chromatographic effluents containing 2-propanol. In the present work it has been demonstrated that low-molecular-weight polycyclic aromatic hydrocarbons can be isolated from reversed-phase HPLC solvents by a one-step solvent extraction using a small volume of solvent. The procedure is rapid and convenient and the use of a small volume of solvent eliminates the need for reducing the volume of extraction solvent by distillation or other techniques. The utility of the method has been demonstrated by the determination of biphenyl on fly ash.
- Research Organization:
- Ames Lab.-USDOE, Iowa State Univ.
- OSTI ID:
- 5194764
- Journal Information:
- Anal. Chem.; (United States), Vol. 54:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
54 ENVIRONMENTAL SCIENCES
ANTHRACENE
SOLVENT EXTRACTION
NAPHTHALENE
POLYCYCLIC AROMATIC HYDROCARBONS
CHROMATOGRAPHY
CONCENTRATION RATIO
EFFICIENCY
EXPERIMENTAL DATA
FLY ASH
AEROSOL WASTES
AROMATICS
ASHES
CONDENSED AROMATICS
DATA
EXTRACTION
HYDROCARBONS
INFORMATION
NUMERICAL DATA
ORGANIC COMPOUNDS
RESIDUES
SEPARATION PROCESSES
WASTES
400105* - Separation Procedures
400102 - Chemical & Spectral Procedures
500200 - Environment
Atmospheric- Chemicals Monitoring & Transport- (-1989)