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Title: Empirical assessment of model validity

Abstract

The metabolism of amino acids is far more complicated than a 1- to 2-pool model. Yet, these simple models have been extensively used with many different isotopically labeled tracers to study protein metabolism. A tracer of leucine and measurement of leucine kinetics has been a favorite choice for following protein metabolism. However, administering a leucine tracer and following it in blood will not adequately reflect the complex multi-pool nature of the leucine system. Using the tracer enrichment of the ketoacid metabolite of leucine, alpha-ketoisocaproate (KIC), to reflect intracellular events of leucine was an important improvement. Whether this approach is adequate to follow accurately leucine metabolism in vivo or not has not been tested. From data obtained using simultaneous administration of leucine and KIC tracers, we developed a 10-pool model of the in vivo leucine-KIC and bicarbonate kinetic system. Data from this model were compared with conventional measurements of leucine kinetics. The results from the 10-pool model agreed best with the simplified approach using a leucine tracer and measurement of KIC enrichment.

Authors:
 [1]
  1. Shriners Burns Institute, Galveston, TX (USA)
Publication Date:
OSTI Identifier:
5190961
Resource Type:
Journal Article
Journal Name:
JPEN, Journal of Parenteral and Enteral Nutrition; (United States)
Additional Journal Information:
Journal Volume: 15:3; Journal ID: ISSN 0148-6071
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; LEUCINE; METABOLISM; CARBON ISOTOPES; MATHEMATICAL MODELS; TRACER TECHNIQUES; VALIDATION; AMINO ACIDS; CARBOXYLIC ACIDS; ISOTOPE APPLICATIONS; ISOTOPES; ORGANIC ACIDS; ORGANIC COMPOUNDS; TESTING; 550501* - Metabolism- Tracer Techniques

Citation Formats

Wolfe, R R. Empirical assessment of model validity. United States: N. p., 1991. Web. doi:10.1177/014860719101500350S.
Wolfe, R R. Empirical assessment of model validity. United States. https://doi.org/10.1177/014860719101500350S
Wolfe, R R. 1991. "Empirical assessment of model validity". United States. https://doi.org/10.1177/014860719101500350S.
@article{osti_5190961,
title = {Empirical assessment of model validity},
author = {Wolfe, R R},
abstractNote = {The metabolism of amino acids is far more complicated than a 1- to 2-pool model. Yet, these simple models have been extensively used with many different isotopically labeled tracers to study protein metabolism. A tracer of leucine and measurement of leucine kinetics has been a favorite choice for following protein metabolism. However, administering a leucine tracer and following it in blood will not adequately reflect the complex multi-pool nature of the leucine system. Using the tracer enrichment of the ketoacid metabolite of leucine, alpha-ketoisocaproate (KIC), to reflect intracellular events of leucine was an important improvement. Whether this approach is adequate to follow accurately leucine metabolism in vivo or not has not been tested. From data obtained using simultaneous administration of leucine and KIC tracers, we developed a 10-pool model of the in vivo leucine-KIC and bicarbonate kinetic system. Data from this model were compared with conventional measurements of leucine kinetics. The results from the 10-pool model agreed best with the simplified approach using a leucine tracer and measurement of KIC enrichment.},
doi = {10.1177/014860719101500350S},
url = {https://www.osti.gov/biblio/5190961}, journal = {JPEN, Journal of Parenteral and Enteral Nutrition; (United States)},
issn = {0148-6071},
number = ,
volume = 15:3,
place = {United States},
year = {Wed May 01 00:00:00 EDT 1991},
month = {Wed May 01 00:00:00 EDT 1991}
}