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Title: Ethanol inhibits human bone cell proliferation and function in vitro

The direct effects of ethanol on human bone cell proliferation and function were studied in vitro. Normal human osteoblasts from trabecular bone chips were prepared by collagenase digestion. Exposure of these osteoblasts to ethanol in concentrations of 0.05% to 1% for 22 hours induced a dose-dependent reduction in bone cell DNA synthesis as assessed by incorporation of 3H-thymidine. After 72 hours of ethanol exposure in concentrations of 0.01% to 1%, protein synthesis as measured by 3H-proline incorporation into trichbroacetic acid (TCA)-precipitable material was reduced in a dose-dependent manner. Human bone cell protein concentrations and alkaline phosphatase total activity were significantly reduced after exposure to 1% ethanol for 72 hours, but not with lower concentrations of ethanol. This reduction in osteoblast proliferation and activity may partially explain the development of osteopenia in humans consuming excessive amounts of ethanol.
Authors:
;  [1]
  1. (University of Washington, Seattle (USA))
Publication Date:
OSTI Identifier:
5185723
Resource Type:
Journal Article
Resource Relation:
Journal Name: Metabolism, Clinical and Experimental; (United States); Journal Volume: 40:6
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; BONE CELLS; CELL PROLIFERATION; ETHANOL; BIOLOGICAL EFFECTS; SKELETAL DISEASES; ETIOLOGY; ALKALINE PHOSPHATASE; DNA REPLICATION; DOSE-RESPONSE RELATIONSHIPS; ENZYME ACTIVITY; MAN; MITOTIC INDEX; PEPTIDE HYDROLASES; PROLINE; PROTEINS; THYMIDINE; TRACER TECHNIQUES; TRITIUM COMPOUNDS; ALCOHOLS; AMINES; AMINO ACIDS; ANIMAL CELLS; ANIMALS; AZINES; AZOLES; CARBOXYLIC ACIDS; CONNECTIVE TISSUE CELLS; DISEASES; ENZYMES; ESTERASES; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; HYDROGEN COMPOUNDS; HYDROLASES; HYDROXY COMPOUNDS; ISOTOPE APPLICATIONS; MAMMALS; NUCLEIC ACID REPLICATION; NUCLEOSIDES; NUCLEOTIDES; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PHOSPHATASES; PRIMATES; PYRIMIDINES; PYRROLES; PYRROLIDINES; RIBOSIDES; SOMATIC CELLS; VERTEBRATES 560300* -- Chemicals Metabolism & Toxicology; 550501 -- Metabolism-- Tracer Techniques