Neomycin resistance as a selectable marker in Methanococcus maripaludis
Abstract
The authors cloned the aminoglycoside phosphotransferase genes APH3{prime}I and APH3{prime}II between the Methanococcus voltae methyl reductase promoter and terminator in a plasmid containing a fragment of Methanococcus maripaludis chromosomal DNA. The resulting plasmids encoding neomycin resistance transformed M. maripaludis at frequencies similar to those observed for pKAS102 encoding puromycin resistance. The antibiotic geneticin was not inhibitory to M. maripaludis. 22 refs., 3 figs., 3 tabs.
- Authors:
-
- Univ. of Washington, Seattle, WA (United States)
- Univ. of Georgia, Athens, GA (United States)
- Publication Date:
- OSTI Identifier:
- 518247
- DOE Contract Number:
- FG05-94ER20158
- Resource Type:
- Journal Article
- Journal Name:
- Applied and Environmental Microbiology
- Additional Journal Information:
- Journal Volume: 62; Journal Issue: 11; Other Information: PBD: Nov 1996
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 55 BIOLOGY AND MEDICINE, BASIC STUDIES; NEOMYCIN; BIOLOGICAL ADAPTATION; BACTERIA; BIOLOGICAL INDICATORS
Citation Formats
Argyle, J L, Leigh, J A, and Tumbula, D L. Neomycin resistance as a selectable marker in Methanococcus maripaludis. United States: N. p., 1996.
Web.
Argyle, J L, Leigh, J A, & Tumbula, D L. Neomycin resistance as a selectable marker in Methanococcus maripaludis. United States.
Argyle, J L, Leigh, J A, and Tumbula, D L. 1996.
"Neomycin resistance as a selectable marker in Methanococcus maripaludis". United States.
@article{osti_518247,
title = {Neomycin resistance as a selectable marker in Methanococcus maripaludis},
author = {Argyle, J L and Leigh, J A and Tumbula, D L},
abstractNote = {The authors cloned the aminoglycoside phosphotransferase genes APH3{prime}I and APH3{prime}II between the Methanococcus voltae methyl reductase promoter and terminator in a plasmid containing a fragment of Methanococcus maripaludis chromosomal DNA. The resulting plasmids encoding neomycin resistance transformed M. maripaludis at frequencies similar to those observed for pKAS102 encoding puromycin resistance. The antibiotic geneticin was not inhibitory to M. maripaludis. 22 refs., 3 figs., 3 tabs.},
doi = {},
url = {https://www.osti.gov/biblio/518247},
journal = {Applied and Environmental Microbiology},
number = 11,
volume = 62,
place = {United States},
year = {Fri Nov 01 00:00:00 EST 1996},
month = {Fri Nov 01 00:00:00 EST 1996}
}
Other availability
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.