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Title: Neomycin resistance as a selectable marker in Methanococcus maripaludis

Abstract

The authors cloned the aminoglycoside phosphotransferase genes APH3{prime}I and APH3{prime}II between the Methanococcus voltae methyl reductase promoter and terminator in a plasmid containing a fragment of Methanococcus maripaludis chromosomal DNA. The resulting plasmids encoding neomycin resistance transformed M. maripaludis at frequencies similar to those observed for pKAS102 encoding puromycin resistance. The antibiotic geneticin was not inhibitory to M. maripaludis. 22 refs., 3 figs., 3 tabs.

Authors:
;  [1];  [2]
  1. Univ. of Washington, Seattle, WA (United States)
  2. Univ. of Georgia, Athens, GA (United States)
Publication Date:
OSTI Identifier:
518247
DOE Contract Number:  
FG05-94ER20158
Resource Type:
Journal Article
Journal Name:
Applied and Environmental Microbiology
Additional Journal Information:
Journal Volume: 62; Journal Issue: 11; Other Information: PBD: Nov 1996
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; NEOMYCIN; BIOLOGICAL ADAPTATION; BACTERIA; BIOLOGICAL INDICATORS

Citation Formats

Argyle, J L, Leigh, J A, and Tumbula, D L. Neomycin resistance as a selectable marker in Methanococcus maripaludis. United States: N. p., 1996. Web.
Argyle, J L, Leigh, J A, & Tumbula, D L. Neomycin resistance as a selectable marker in Methanococcus maripaludis. United States.
Argyle, J L, Leigh, J A, and Tumbula, D L. 1996. "Neomycin resistance as a selectable marker in Methanococcus maripaludis". United States.
@article{osti_518247,
title = {Neomycin resistance as a selectable marker in Methanococcus maripaludis},
author = {Argyle, J L and Leigh, J A and Tumbula, D L},
abstractNote = {The authors cloned the aminoglycoside phosphotransferase genes APH3{prime}I and APH3{prime}II between the Methanococcus voltae methyl reductase promoter and terminator in a plasmid containing a fragment of Methanococcus maripaludis chromosomal DNA. The resulting plasmids encoding neomycin resistance transformed M. maripaludis at frequencies similar to those observed for pKAS102 encoding puromycin resistance. The antibiotic geneticin was not inhibitory to M. maripaludis. 22 refs., 3 figs., 3 tabs.},
doi = {},
url = {https://www.osti.gov/biblio/518247}, journal = {Applied and Environmental Microbiology},
number = 11,
volume = 62,
place = {United States},
year = {Fri Nov 01 00:00:00 EST 1996},
month = {Fri Nov 01 00:00:00 EST 1996}
}