Influence of chemical and environmental stresses on metal-binding proteins: Species-dependent effects

Thesis/Dissertation · Fri Jan 01 00:00:00 EST 1988

OSTI ID:5181712

Baer, K N

The development of tolerance to cadmium toxicity was investigated in mammals. In adult mice pretreated with 20 mg Cd/kg, no mortality was observed following administration of a 100 mg/kg cadmium challenge dose. In animals receiving prior exposure to cold stress a mortality of 40% was observed, while in animals receiving no pretreatment an 80% mortality was observed following cadmium challenge. Analysis of the metal-binding proteins using G-75 gel-filtration chromatography revealed that MT-like protein was responsible, in part, for the observed tolerance to cadmium toxicity. For example, following 20 mg Cd/kg and cold pretreatment, the MT-like reserve capacity was 56 and 42 nmoles cadmium, respectively, compared to a control value of 12 nmoles cadmium. The influence of pretreatments on the subcellular distribution of cadmium was also examined. The influence of chemical and environmental stresses on metal-binding proteins in teleosts was investigated. Following cadmium exposure, cadmium increased in the MT fraction in both the gill and liver. However, following exposure to environmental stresses such as cold and hypoxia, significant decreases in zinc and copper were observed in the gill MT fraction, as compared to control. In the liver, no significant alterations were observed in the MT fraction, as compared to control.

• Research Organization: Northeast Louisiana Univ., Monroe, LA (USA)
• OSTI ID: 5181712
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CADMIUM
TOXICITY
HYPOTHERMIA
BIOLOGICAL EFFECTS
METALLOPROTEINS
BIOSYNTHESIS
BIOLOGICAL STRESS
CHROMATOGRAPHY
FISHES
GENETIC VARIABILITY
MICE
MORTALITY
SUBCELLULAR DISTRIBUTION
TEMPERATURE EFFECTS
ANIMALS
AQUATIC ORGANISMS
BIOLOGICAL VARIABILITY
BODY TEMPERATURE
DISTRIBUTION
ELEMENTS
MAMMALS
METALS
ORGANIC COMPOUNDS
PROTEINS
RODENTS
SEPARATION PROCESSES
SYNTHESIS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
560200 - Thermal Effects