Aldosterone binding in isolated tubules. IV. Autoradiography along the nephron of the spontaneously hypertensive rat
The binding of aldosterone was studied in tubular segments isolated by microdissection from kidneys of spontaneously hypertensive (SHR, n = 8), Kyoto normotensive (KWR, n = 8), and normal Wistar (NWR, n = 6) rats with an autoradiographic technique on dry film. All animals had been previously adrenalectomized. Kidney pyramids were incubated in vitro before microdissection with collagenase and 2 X 10(-9) M (/sup 3/H)aldosterone in the presence or absence of an excess of unlabeled aldosterone. In addition, the displacement of the binding by 10 times excess dexamethasone or aldosterone was examined in the cortical and medullary collecting tubule of SHR and KWR to assess the specificity of binding sites. In the three groups, no specific nuclear labeling was detectable in the proximal tubule. The highest specific nuclear labeling was found in the distal portions of the nephron, and intermediate values were present along the loop of Henle. In the cortical collecting tubule, the most specific mineralocorticoid segment, the specific nuclear binding, expressed in silver grains per unit surface, was significantly elevated in SHR (16.1 +/- 1.5) and KWR (13.7 +/- 1.5) as compared with NWR (10.2 +/- 0.8, P less than 0.001 and less than 0.05, respectively). The difference between SHR and KWR did not reach statistical significance. In the medullary collecting tubule, binding was higher in SHR (14.3 +/- 1.6) than in both KWR (8.7 +/- 1.0, P less than 0.005) and NWR (10.1 +/- 0.9, P less than 0.025).
- Research Organization:
- Institut National de la Sante et de la Recherche Medicale, Saclay, France
- OSTI ID:
- 5180258
- Journal Information:
- Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States)
- Country of Publication:
- United States
- Language:
- English
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ALDOSTERONE
CHEMICAL BONDS
KIDNEYS
AUTORADIOGRAPHY
ADRENAL GLANDS
ADRENALECTOMY
COMPARATIVE EVALUATIONS
DEXAMETHASONE
HYDROLASES
HYPERTENSION
RATS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ADRENAL HORMONES
ALDEHYDES
ANIMALS
BODY
CARDIOVASCULAR DISEASES
CORTICOSTEROIDS
DISEASES
ENDOCRINE GLANDS
ENZYMES
GLANDS
GLUCOCORTICOIDS
HORMONES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KETONES
LABELLED COMPOUNDS
MAMMALS
MEDICINE
MINERALOCORTICOIDS
ORGANIC COMPOUNDS
ORGANS
PREGNANES
RODENTS
STEROID HORMONES
STEROIDS
SURGERY
SYMPTOMS
VASCULAR DISEASES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques