Uterine type II estrogen-binding sites are not of eosinophil origin

Journal Article · Sun Jan 05 00:00:00 EST 1986 · J. Biol. Chem.; (United States)

OSTI ID:5179183

A recent report suggested that nuclear type II sites in the rat uterus are of eosinophil origin and may represent (/sup 3/H)estradiol binding to eosinophil peroxidase. To further evaluate this hypothesis the authors examined the response of nuclear type II sites to estrogen under conditions where eosinophils are not present. Results of the experiments show that physiological levels of estradiol-17..beta.. (10 nM for 72 h) will stimulate nuclear type II sites in highly purified cultures of rat uterine stromal and myometrial cells. The magnitude of the response of type II sites to estradiol in these stromal (4-fold) and myometrial (80-fold) cell cultures was essentially identical to that observed in the uterine cell types following in vivo estrogen treatment. Since these highly purified cultures of uterine cells were prepared from the uterus of a 21-day ovariectomized rat which is devoid of eosinophils, it was concluded that estradiol stimulation of nuclear type II sites is a direct intracellular response to estrogen which occurs independent of eosinophil accumulation. Furthermore, it was found that type II sites in the rat uterus are not peroxidase. Stimulation of cytosol and nuclear type II sites by estrogen in the rat uterus is a direct intracellular response to the hormone unrelated to eosinophil accumulation and/or peroxidase activity.

• Research Organization: Baylor College of Medicine, Houston, TX
• OSTI ID: 5179183
• Journal Information: J. Biol. Chem.; (United States), Vol. 261:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
EOSINOPHILS
ESTRADIOL
BIOCHEMICAL REACTION KINETICS
UTERUS
ANIMAL CELLS
CELL CULTURES
EXPERIMENTAL DATA
IN VIVO
PEROXIDASES
RATS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
DATA
ENZYMES
ESTRANES
ESTROGENS
FEMALE GENITALS
HORMONES
HYDROXY COMPOUNDS
INFORMATION
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LEUKOCYTES
MAMMALS
MATERIALS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
REACTION KINETICS
RODENTS
STEROID HORMONES
STEROIDS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques