DNA unwinding proteins of an eukaryotic organism. Final report, July 1977-April 1981

Technical Report · Tue Jun 01 00:00:00 EDT 1982

OSTI ID:5178134

Patel, G L

A rat liver protein, which preferentially binds in vitro to single-stranded DNA, and destabilizes the DNA double helix to promote its unwinding or melting was investigated. Proteins of this class isolated from viral, prokaryotic, and eukaryotic sources have been referred to as DNA melting, unwinding, helix-destabilizing, or single-strand binding proteins. The rat liver protein that we have purified and studied here is referred to as a helix-destabilizing protein (HDP). The specific objectives proposed were to: (1) purify and characterize physico-chemically one or more rat liver HDP; (2) prepare specific anti-HDP sera for immunological approaches; (3) characterize the HDP interaction with DNA; and (4) study the HDP metabolism in normal and regenerating liver. A novel conceptual contribution was made with regards to the biological roles of this class of proteins. Activities and accomplishments during this entire contract period are summarized. (ERB)

• Research Organization: Georgia Univ., Athens (USA). Dept. of Zoology
• DOE Contract Number: AS09-76EV00644
• OSTI ID: 5178134
• Report Number(s): DOE/EV/00644-22; ON: DE82018596
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DNA
STABILITY
PROTEINS
CHEMICAL BONDS
METABOLISM
PURIFICATION
CHEMICAL PROPERTIES
HELICAL CONFIGURATION
IMMUNE SERUMS
IMMUNOLOGY
LIVER
PHYSICAL PROPERTIES
RATS
STRUCTURAL CHEMICAL ANALYSIS
ANIMALS
BODY
CONFIGURATION
DIGESTIVE SYSTEM
GLANDS
MAMMALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RODENTS
VERTEBRATES
550200* - Biochemistry
551000 - Physiological Systems