Protection from radiation-induced enteropathy by elemental diet feeding: The role of free radicals
- McGill Univ., Montreal, Quebec (Canada)
Free radicals have been implicated in intestinal reperfusion injury following ischemia and in epithelial cell damage resulting from ionizing radiation. Elemental diets (ED) have been shown to afford significant prophylaxis to the intestine from these injuries. The purpose of the present study was to investigate whether ED alters the activity of the defense mechanisms necessary for free radical removal. Six female dogs, fed on normal dog chow, had a 30 cm resection of terminal ileum to form Thiry-Vella loops. The main intestine was biopsied and anastomosed. Two weeks later, biopsies were taken from the lips of the loops. Following this, the loops were fed daily with ED another 2 weeks and biopsied again. The dogs were then placed on ED for 3 days before and during 4 days of pelvic irradiation, and the loops also were fed ED daily; after which the animals were again anesthetized, and the loops and main intestine were biopsied. All biopsies were processed for histology, and assayed for xanthine oxidase (XO), superoxide dismutase (SOD), glutathione peroxidase (GSP) and catalase (CAT). The XO and SOD pathway of free oxygen radical generation and scavenging are not affected by radiation. However, ED lowers both XO and SOD activity and may result in a reduced production of peroxides. The significantly increased activity of GSP and CAT when ED is fed improves the scavenging capacity of the free hydroxyl radicals generated by the radiation, and is an important adjunct to an understanding of ED prophylaxis.
- OSTI ID:
- 5175141
- Report Number(s):
- CONF-9104107-; CODEN: FAJOE
- Journal Information:
- FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 5:5; Conference: 75. annual meeting of the Federation of American Societies for Experimental Biology (FASEB), Atlanta, GA (United States), 21-25 Apr 1991; ISSN 0892-6638
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DIET
RADIOSENSITIVITY EFFECTS
DIGESTIVE SYSTEM DISEASES
RADIOINDUCTION
OXIDOREDUCTASES
ENZYME ACTIVITY
RADICALS
TOXICITY
BIOLOGICAL FUNCTIONS
BIOLOGICAL PATHWAYS
BIOLOGICAL RADIATION EFFECTS
BIOPSY
CATALASE
DOGS
INTESTINES
IONIZING RADIATIONS
PEROXIDASES
SUPEROXIDE DISMUTASE
ANIMALS
BIOLOGICAL EFFECTS
BODY
DIAGNOSTIC TECHNIQUES
DIGESTIVE SYSTEM
DISEASES
ENZYMES
GASTROINTESTINAL TRACT
MAMMALS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIATION EFFECTS
RADIATIONS
VERTEBRATES
560152* - Radiation Effects on Animals- Animals