Differential regulation by phorbol ester of formyl-methionyl peptide and leukotriene B sub 4 receptors on human neutrophils
- John Hopkins Univ., Baltimore, MD (United States)
Activation of protein kinase C (PKC) with suboptimal does of phorbol myristyl acetate (PMA) will increase fMP receptor expression with parallel potentiation of superoxide generation. PMA-induced changes in leukotriene B{sub 4} (LTB{sub 4}) receptor expression were assessed in parallel with fMP receptor expression to determine if these two independent receptor classes are regulated in a similar manner by PKC. The relative density of fMP receptors was assessed by flow cytometry. The relative density of receptors for LTB{sub 4} was quantitated by incubating 2 {times} 10{sup 6} Ns with 10nM({sup 3}H)-LTB{sub 4} and determining the amount of radioactivity bound after filtration on glass fiber filters. Incubation of N with 10ng/mL PMA induced a 3.2-fold increase in fMP receptor expression by 5 min which was sustained for up to 15 min. In contrast, LTB{sub 4} receptor density decreased by 36% within 5 min. in response to 10 ng/mL PMA. Staurosporine, a potent antagonist of PKC, had no effect of fMP receptor expression but markedly enhanced LTB{sub 4} receptor expression by 1.7-fold at 200nM. PKC acts to decrease the surface expression of LTB{sub 4} receptors in contrast to the enhancement of fMP receptor expression, suggesting in contrast to the enhancement of fMP receptor expression, suggesting that potentiation of N function by PMA may be stimulus-specific.
- OSTI ID:
- 5172731
- Report Number(s):
- CONF-9104107-; CODEN: FAJOE
- Journal Information:
- FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 5:5; Conference: 75. annual meeting of the Federation of American Societies for Experimental Biology (FASEB), Atlanta, GA (United States), 21-25 Apr 1991; ISSN 0892-6638
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
PEPTIDES
RECEPTORS
PHORBOL ESTERS
BIOLOGICAL EFFECTS
PHOSPHOTRANSFERASES
ENZYME ACTIVITY
PROSTAGLANDINS
CELL FLOW SYSTEMS
MAN
NEUTROPHILS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARCINOGENS
ENZYMES
ESTERS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
LEUKOCYTES
MAMMALS
MATERIALS
MEMBRANE PROTEINS
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PRIMATES
PROTEINS
TRANSFERASES
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology