Morphological changes in cerebellum of neonatal rats exposed to 2. 45 GHz microwaves
One-day and six-day old Sprague-Dawley rats were exposed in the far field to 2.45 GHz (cw) microwaves at 10 mW/cm/sup 2/ for five consecutive days, 7 hours per day (SAR 2W/kg). Pups were euthenized one day after exposure and the cerebella processed for light and electron microscopy. Matching cerebellar sections and folia from irradiated and sham irradiated animals were examined. Light microscopic examination revealed the presence of small deeply-stained cells with hyperchromatic pyknotic nuclei within the external granular layer (EGL). The number of these pyknotic cells in the experimental animals was nearly twice that in the controls. The Nissl bodies in Purkinje cells were finely dispersed. In some experimental animals mononuclear cellular infiltration was demonstrated. Under the electron microscope the deeply-stained pyknotic small cells presented electron dense nuclei with clumped chromatin, extrusion or disintegration of the nucleus, ruptured nuclear membrane, and the vacuolization of the cytoplasm. Eventually these cells became phagocytosed by surrounding EGL cells. Most of the Purkinje cells of experimental animals showed small, disorderly arrays of rough endoplasmic reticulum (RER) instead of the typical orderly stacks of parallel arrays. These observations suggest that microwave radiation may interfere with early genesis of cerebellar microneurons and alter the metabolic status of Purkinje cells. However, this effect might be reversible.
- Research Organization:
- George Washington Univ. Medical Center, Washington, DC (USA)
- OSTI ID:
- 5163891
- Journal Information:
- Prog. Clin. Biol. Res.; (United States), Vol. 257
- Country of Publication:
- United States
- Language:
- English
Similar Records
Effects of 2. 45 GHz microwave radiation on the development of Japanese quail cerebellum
Histological changes during development of the cerebellum in the chick embryo exposed to a static magnetic field
Related Subjects
CEREBELLUM
MORPHOLOGICAL CHANGES
MICROWAVE RADIATION
BIOLOGICAL EFFECTS
BLOOD-BRAIN BARRIER
CELL MEMBRANES
CELL NUCLEI
CYTOPLASM
ELECTRON MICROSCOPY
ENDOPLASMIC RETICULUM
NEONATES
RATS
ANIMALS
BODY
BRAIN
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM
ELECTROMAGNETIC RADIATION
MAMMALS
MEMBRANES
MICROSCOPY
NERVOUS SYSTEM
ORGANOIDS
ORGANS
RADIATIONS
RODENTS
VERTEBRATES
560400* - Other Environmental Pollutant Effects