cDNA cloning and expression of the human A-type platelet-derived growth factor (PDGF) receptor establishes structural similarity to the B-type PDGF receptor
- Ludwig Institute for Cancer Research, Uppsala (Sweden)
The primary structure of the human A-type receptor for platelet-derived growth factor (PDGF) has been determined. A 6.5-kilobase (kb) transcript was identified through low-stringency hybridization with a probe derived from the B-type PDGF receptor cDNA. The sequence of a cDNA clone corresponding to the 6.5-kb transcript contains an open reading frame that predicts a 1,089-amino acid growth factor receptor-like molecule, which displays 44% overall amino acid similarity with the PDGF B-type receptor. The two receptors have a similar domain organization, with five immunoglobulin-like domains extracellularly and an intracellular split protein tyrosine kinase domain. Transfection of the new cDNA into COS cells led to the expression of a protein specifically recognized by an antiserum previously shown to react with the PDGF A-type receptor. The expressed protein was shown to display high-affinity binding of all three {sup 125}I-labeled dimeric forms of PdGF A and B chains in a manner that is characteristic for the PDGF A-type receptor.
- OSTI ID:
- 5161018
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 86:13; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
Similar Records
Cloning and expression of a cDNA coding for the human platelet-derived growth factor receptor: Evidence for more than one receptor class
Cloning and expression of cDNAs for two distinct murine tumor necrosis factor receptors demonstrate one receptor is species specific
Related Subjects
GROWTH FACTORS
RADIORECEPTOR ASSAY
RECEPTORS
MOLECULAR STRUCTURE
RECOMBINANT DNA
DNA-CLONING
BLOOD PLATELETS
CYSTEINE
DNA SEQUENCING
IODINE 125
MAN
MEMBRANE PROTEINS
MEMBRANES
METHIONINE
PROTEINS
SULFUR 35
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CLONING
DAYS LIVING RADIOISOTOPES
DNA
DNA HYBRIDIZATION
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
HYBRIDIZATION
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAMMALS
MATERIALS
MITOGENS
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PRIMATES
RADIOISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
SULFUR ISOTOPES
THIOLS
TRACER TECHNIQUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques