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Title: Comparison of fluorographic methods for detecting radioactivity in polyacrylamide gels or on nitrocellulose filters

Abstract

The commercial fluorographic enhancers, En3Hance or Amplify, were not as efficient as 2,5-diphenyloxazole (PPO) for detecting radioactively labeled proteins in polyacrylamide gels or on nitrocellulose filters. For most of the X-ray films tested, optimal preexposure was essential to obtain maximum sensitivity in fluorography or indirect autoradiography using intensifying screens. The best results were obtained with nitrocellulose by saturating the filters with PPO. The minimum levels of /sup 35/S//sup 14/C that could be detected on filters by autoradiography or fluorography in a 24-h exposure were 4 X 10(2) or 1 X 10(2) dpm cm-2 respectively. For /sup 3/H these levels were, respectively, 20 X 10(3) or 0.5 X 10(3) dpm cm-2.

Authors:
Publication Date:
Research Org.:
Univ. of Leeds, England
OSTI Identifier:
5151531
Resource Type:
Journal Article
Journal Name:
Anal. Biochem.; (United States)
Additional Journal Information:
Journal Volume: 2
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; PROTEINS; AUTORADIOGRAPHY; AZOLES; CARBON 14 COMPOUNDS; COMPARATIVE EVALUATIONS; FILTERS; FILTRATION; FLUORESCENCE; NITROCELLULOSE; SULFUR 35; TRITIUM COMPOUNDS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CARBOHYDRATES; CELLULOSE ESTERS; CHEMICAL EXPLOSIVES; DAYS LIVING RADIOISOTOPES; ESTERS; EVEN-ODD NUCLEI; EXPLOSIVES; HETEROCYCLIC COMPOUNDS; ISOTOPES; LABELLED COMPOUNDS; LIGHT NUCLEI; LUMINESCENCE; NITRIC ACID ESTERS; NUCLEI; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; POLYSACCHARIDES; RADIOISOTOPES; SACCHARIDES; SEPARATION PROCESSES; SULFUR ISOTOPES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Roberts, P L. Comparison of fluorographic methods for detecting radioactivity in polyacrylamide gels or on nitrocellulose filters. United States: N. p., 1985. Web. doi:10.1016/0003-2697(85)90308-2.
Roberts, P L. Comparison of fluorographic methods for detecting radioactivity in polyacrylamide gels or on nitrocellulose filters. United States. https://doi.org/10.1016/0003-2697(85)90308-2
Roberts, P L. 1985. "Comparison of fluorographic methods for detecting radioactivity in polyacrylamide gels or on nitrocellulose filters". United States. https://doi.org/10.1016/0003-2697(85)90308-2.
@article{osti_5151531,
title = {Comparison of fluorographic methods for detecting radioactivity in polyacrylamide gels or on nitrocellulose filters},
author = {Roberts, P L},
abstractNote = {The commercial fluorographic enhancers, En3Hance or Amplify, were not as efficient as 2,5-diphenyloxazole (PPO) for detecting radioactively labeled proteins in polyacrylamide gels or on nitrocellulose filters. For most of the X-ray films tested, optimal preexposure was essential to obtain maximum sensitivity in fluorography or indirect autoradiography using intensifying screens. The best results were obtained with nitrocellulose by saturating the filters with PPO. The minimum levels of /sup 35/S//sup 14/C that could be detected on filters by autoradiography or fluorography in a 24-h exposure were 4 X 10(2) or 1 X 10(2) dpm cm-2 respectively. For /sup 3/H these levels were, respectively, 20 X 10(3) or 0.5 X 10(3) dpm cm-2.},
doi = {10.1016/0003-2697(85)90308-2},
url = {https://www.osti.gov/biblio/5151531}, journal = {Anal. Biochem.; (United States)},
number = ,
volume = 2,
place = {United States},
year = {Sat Jun 01 00:00:00 EDT 1985},
month = {Sat Jun 01 00:00:00 EDT 1985}
}