Case study in process development: Production of human interleukin-3 using industrial microorganisms

Journal Article · Tue Jan 01 00:00:00 EST 1991 · Bio/Technology; (United States)

DOI:https://doi.org/10.1038/nbt0191-47· OSTI ID:5148299

Leen, R.W. van; Bakhuis, J G; Hommes, R W.J.; Lemson, P J; Noordam, B; Persoon, N L.M. ^[1]; Burger, H ^[2]; Dorssers, L C.J. ^[3]; Wagemaker, G ^[4]

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1. Royal Gist-brocades NV, t (Netherlands)
2. ITRI-TNO, Rijswijk (Netherlands)
3. Dr. Daniel den Hoed Kliniek, Rotterdam (Netherlands)
4. Erasmus Univ., Rotterdam (Netherlands) ITRI-TNO, Rijswijk (Netherlands)

The authors expressed a cDNA encoding the multi-colony stimulating factor interleukin-3 in a variety of cell types, including bacteria, yeast and mammalian cells. After evaluation of the advantages and disadvantages of each potential system, they designed a production and purification scheme using Bacillus licheniformis. The purification consists of hydrophobic interaction chromatography, two steps of ion exchange chromatography and gel filtration. The purified and formulated product entered clinical trials in November 1989.

• OSTI ID: 5148299
• Journal Information: Bio/Technology; (United States), Vol. 9:1; ISSN 0733-222X
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BACILLUS LICHENIFORMIS
GENETIC ENGINEERING
GROWTH FACTORS
PURIFICATION
ANIMAL CELLS
CELL DIFFERENTIATION
ION EXCHANGE CHROMATOGRAPHY
RECOMBINANT DNA
STEM CELLS
YEASTS
BACILLUS
BACTERIA
BIOTECHNOLOGY
CHROMATOGRAPHY
DNA
EUMYCOTA
FUNGI
MICROORGANISMS
MITOGENS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PLANTS
PROTEINS
SEPARATION PROCESSES
SOMATIC CELLS
550200* - Biochemistry
550400 - Genetics