Arabidopsis ethylene-response gene ETR1: Similiarity of product to two-component regulators

Journal Article · Fri Oct 22 00:00:00 EDT 1993 · Science (Washington, D.C.); (United States)

DOI:https://doi.org/10.1126/science.8211181· OSTI ID:5147310

Chang, C; Kwok, S F; Bleecker, A B; Meyerowitz, E M ^[1]

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1. California Institute of Technology, Pasadena, CA (United States)

Ethylene behaves as a hormone in plants, regulating such aspects of growth and development as fruit ripening, flower senescence, and abscission. Ethylene insensitivity is conferred by dominant mutations in the ETR1 gene early in the ethylene signal transduction pathway of Arabidopsis thaliana. The ETR1 gene was cloned by the method of chromosome walking. Each of the four known etr1 mutant alleles contains a missense mutation near the amino terminus of the predicted protein. Although the sequence of the amino-terminal half of the deduced ETR1 protein appears to be novel, the carboxyl-terminal half is similar in sequence to both components of the prokaryotic family of signal transducers known as the two-component systems. Thus, an early step in ethylene signal transduction in plants may involve transfer of phosphate as in prokaryotic two-component systems. The dominant etr1-1 mutant gene conferred ethylene insensitivity to wild-type Arabidopsis plants when introduced by transformation.

• OSTI ID: 5147310
• Journal Information: Science (Washington, D.C.); (United States), Vol. 262:5133; ISSN 0036-8075
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ETHYLENE
BIOLOGICAL EFFECTS
GENES
DOMINANT MUTATIONS
AMINO ACID SEQUENCE
DNA SEQUENCING
DNA-CLONING
PLANT GROWTH REGULATORS
ALKENES
CLONING
DNA HYBRIDIZATION
HYBRIDIZATION
HYDROCARBONS
MOLECULAR STRUCTURE
MUTATIONS
ORGANIC COMPOUNDS
STRUCTURAL CHEMICAL ANALYSIS
550400* - Genetics
550200 - Biochemistry