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Title: Optimization of batch alcoholic fermentation of glucose syrup substrate. [Box-Wilson central composite design]

The quantitative effects of substrate concentration, yeast concentration, and nutrient supplementation on ethanol content, fermentation time, and ethanol productivity were investigated in a Box-Wilson central composite design experiment, consisting of five levels of each variable. The highest ethanol productivity of about 21 g EtOH/liter hr was obtained at low substrate concentration (i.e., 12/degree/Brix), low alcohol content (i.e., 6% by weight), high yeast concentration (i.e., 4.4%), and high supplementation of yeast extract (i.e., 6% by weight), high yeast concentration (i.e., 4.4%), and high supplementation of yeast extract (i.e., 2.8%). Productivity of this magnitude is substantially higher than that of the traditional batch fermentation or fed-batch fermentation. It is comparable to the results of continuous fermentation but lower than those of vacuum fermentation. Optimal conditions for maximal ethanol productivity can be established by a multiple regression analysis technique and by plotting the contours of constant response to conform to the constraints of individual operations. 12 refs.
Authors:
Publication Date:
OSTI Identifier:
5135443
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biotechnol. Bioeng.; (United States); Journal Volume: 23:8
Research Org:
Univers Foods Corp, Milwaukee, Wis, USA
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; ETHANOL; PRODUCTION; FERMENTATION; OPTIMIZATION; GLUCOSE; SUBSTRATES; YEASTS; ALCOHOLS; ALDEHYDES; BIOCONVERSION; CARBOHYDRATES; FUNGI; HEXOSES; HYDROXY COMPOUNDS; MICROORGANISMS; MONOSACCHARIDES; ORGANIC COMPOUNDS; PLANTS; SACCHARIDES 090222* -- Alcohol Fuels-- Preparation from Wastes or Biomass-- (1976-1989); 140504 -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)