Insulin-like growth factors (IGFs) as autocrine/paracrine regulators of granulosa cell differentiation and growth: Studies with a neutralizing monoclonal antibody to IGF-I

Journal Article · Sat Jul 01 00:00:00 EDT 1989 · Biology of Reproduction; (USA)

DOI:https://doi.org/10.1095/biolreprod41.1.79· OSTI ID:5132462

Mondschein, J S; Canning, S F; Miller, D Q; Hammond, J M ^[1]

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1. Pennsylvania State Univ., Hershey (USA)

Evidence that granulosa cells secrete and respond to insulin-like growth factors (IGFs) suggests, but does not prove, the importance of IGFs as intraovarian regulators. To further assess the role of these peptides in ovarian function, a neutralizing monoclonal antibody to IGF-I was employed to block the actions of IGFs in porcine follicular fluid and in granulosa cell-conditioned medium. In one series of experiments, granulosa cells from immature porcine follicles were cultured in medium containing porcine follicular fluid that had been charcoal-treated to remove steroids. As noted before, fluid from large follicles (LFF) stimulated progesterone production in a dose-dependent manner. The stimulatory effect of LFF (30% v/v) could be inhibited by greater than 50% by the anti-IGF monoclonal antibody. This inhibitory action was specific for the anti-IGF antibody and could be overcome by the addition of excess exogenous IGFs. In another series of experiments, granulosa cells were made dependent on endogenously produced IGFs by culturing them in a serum-free medium without exogenous growth factors. The effects of follicle-stimulating hormone (FSH), estradiol (E2), growth hormone (GH), and combinations thereof on progesterone production were inhibited by approximately 50% by the anti-IGF antibody. The effects of IGFs on indices of cell growth (judged by the criterion of being inhibited by the anti-IGF antibody) were less dramatic. A modest 18% increase in cell number was observed with FSH and E2 treatment in serum-free medium; this effect was virtually abolished by the antibody.

• OSTI ID: 5132462
• Journal Information: Biology of Reproduction; (USA), Vol. 41:1; ISSN 0006-3363
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GROWTH FACTORS
BIOCHEMICAL REACTION KINETICS
LEUKOCYTES
CELL DIFFERENTIATION
OVARIES
BIOLOGICAL FUNCTIONS
PROGESTERONE
BIOSYNTHESIS
DOSE-RESPONSE RELATIONSHIPS
ESTRADIOL
FSH
INHIBITION
INSULIN
MONOCLONAL ANTIBODIES
STH
SWINE
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMALS
ANTIBODIES
AZINES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
DOMESTIC ANIMALS
ESTRANES
ESTROGENS
FEMALE GENITALS
FUNCTIONS
GONADOTROPINS
GONADS
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KETONES
KINETICS
MAMMALS
MATERIALS
MITOGENS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
PREGNANES
PROTEINS
PYRIMIDINES
REACTION KINETICS
RIBOSIDES
STEROID HORMONES
STEROIDS
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques