Molecular analysis of radiation-induced albino (c)-locus mutations that cause death at preimplantation stages of development

Journal Article · Wed Dec 01 00:00:00 EST 1993 · Genetics; (United States)

OSTI ID:5131024

Rinchik, E M ^[1]; Toenjes, R R; Paul, D ^[2]; Potter, M D ^[3]

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1. Oak Ridge National Lab., TN (United States)
2. Fraunhofer-Instituet fuer Toxikologie und Aerosolforschung, Hannover (Germany)
3. Univ. of Tenn.-Oak Ridge Graduate School of Biomedical Sciences, Oak Ridge, TN (United States)

Deletion mutations at the albino (c) locus have been useful for continuing the development of fine-structure physical and functional maps of the Fes-Hbb region of mouse chromosome 7. This report describes the molecular analysis of a number of radiation-induced c deletions that, when homozygous, cause death of the embryo during preimplantation stages. The distal extent of these deletions defines a locus, pid, (preimplantation development) genetically associated with this phenotype. The proximal breakpoints of eight of these deletions were mapped with respect to the Tyr (tyrosinase; albino) gene as well as to anonymous loci within the Fah-Tyr region that are defined by the Pmv-31 viral integration site and by chromosome-microdissection clones. Rearrangements corresponding to the proximal breakpoints of two of these deletions were detected by Southern blot analysis, and a size-altered restriction fragment carrying the breakpoint of one of them was cloned. A probe derived from this deletion fusion fragment defines a locus, D7Rn6, which maps within (or distal to) the pid region, and which discriminates among the distal extents of deletions eliciting the pid phenotype. Extension of physical maps from D7Rn6 should provide access both to the pid region and to loci mapping distal to pid that are defined by N-ethyl-N-nitrosourea-induced lethal mutations. 36 refs., 10 figs.

• DOE Contract Number: AC05-84OR21400
• OSTI ID: 5131024
• Journal Information: Genetics; (United States), Vol. 135:4; ISSN 0016-6731
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
IONIZING RADIATIONS
GENETIC RADIATION EFFECTS
LETHAL MUTATIONS
GENETIC MAPPING
MICE
ONTOGENESIS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
GENETIC EFFECTS
MAMMALS
MAPPING
MUTATIONS
RADIATION EFFECTS
RADIATIONS
RODENTS
VERTEBRATES
560152* - Radiation Effects on Animals- Animals
550400 - Genetics