Subcellular localization and paramagnetic properties of signals observed in Krebs II ascites cells by electron spin resonance spectroscopy

Journal Article · Tue Apr 01 00:00:00 EST 1980 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3575235· OSTI ID:5130921

Lloveras, J ^[1]; Vincensini, P; Ribbes, G; Record, M; Ferre, G; Douste-Blazy, L; Pescia, J

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1. INSERM, Toulouse, France

Subcellular fractions of Krebs II ascites cells were examined by Electron Spin Resonance spectroscopy. Three signals were observed: (1) one at g = 2.005 associated with organic free radicals; (2) another at g = 2.01 formed by three peaks with a hyperfine splitting of 16 G; and (3) a third at g = 2.03, observed only in the particulate fraction 40,000 x g (30 min) and in the cytosol. This latter signal, of endogenous origin, seems similar to the one which is assigned in the literature to dinitrosyl-non-hem-iron complexes, and the role of such complexes in the carcinogenic process is often emphasized. Therefore, Krebs II ascites cells appear to be a useful model for investigating the endogenous 2.03 complex in relation to its localization and nature.

• OSTI ID: 5130921
• Journal Information: Radiat. Res.; (United States), Vol. 82:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ASCITES TUMOR CELLS
ELECTRON SPECTROSCOPY
BIOCHEMISTRY
ELECTRON SPIN RESONANCE
BIOLOGICAL LOCALIZATION
BIOLOGICAL MODELS
CELL CONSTITUENTS
CYTOLOGY
RADICALS
ANIMAL CELLS
BIOLOGY
CHEMISTRY
MAGNETIC RESONANCE
RESONANCE
SPECTROSCOPY
TUMOR CELLS
550300* - Cytology
550200 - Biochemistry
550301 - Cytology- Tracer Techniques