RNA sequence and transcriptional properties of the 3' end of the Newcastle disease virus genome

Journal Article · Sun Sep 01 00:00:00 EDT 1985 · Virology; (United States)

DOI:https://doi.org/10.1016/0042-6822(85)90154-0· OSTI ID:5127262

Kurilla, M G; Stone, H O; Keene, J D

The 3' end of the genomic RNA of Newcastle disease virus (NDV) has been sequenced and the leader RNA defined. Using hybridization to a 3'-end-labeled genome, leader RNA species from in vitro transcription reactions and from infected cell extracts were found to be 47 and 53 nucleotides long. In addition, the start site of the 3'-proximal mRNA was determined by sequence analysis of in vitro (beta-32P)GTP-labeled transcription products. The genomic sequence extending beyond the leader region demonstrated an open reading frame for at least 42 amino acids and probably represents the amino terminus of the nucleocapsid protein (NP). The terminal 8 nucleotides of the NDV genome were identical to those of measles virus and Sendai virus while the sequence of the distal half of the leader region was more similar to that of vesicular stomatitis virus. These data argue for strong evolutionary relatedness between the paramyxovirus and rhabdovirus groups.

• Research Organization: Duke Univ. Medical Center, Durham, NC
• OSTI ID: 5127262
• Journal Information: Virology; (United States), Vol. 2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
RNA
HYBRIDIZATION
AMINO ACID SEQUENCE
GENETICS
IN VITRO
LABELLED COMPOUNDS
MESSENGER-RNA
NUCLEOTIDES
PHOSPHORUS 32
TRACER TECHNIQUES
TRANSCRIPTION
VIRAL DISEASES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGY
DAYS LIVING RADIOISOTOPES
DISEASES
INFECTIOUS DISEASES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
RADIOISOTOPES
550401* - Genetics- Tracer Techniques