RPD1 (SIN3/UME4) is required for maximal activation and repression of diverse yeast genes

Journal Article · Sun Dec 01 00:00:00 EST 1991 · Molecular and Cellular Biology; (United States)

DOI:https://doi.org/10.1128/MCB.11.12.6306· OSTI ID:5118078

Vidal, M; Gaber, R F ^[1]; Strich, R; Esposito, R E ^[2]

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1. Northwestern Univ., Evanston, IL (United States)
2. Univ. of Chicago, IL (United States)

The authors show that the extent of transcriptional regulation of many, apparently unrelated, genes in Saccharomyces cerevisiae is dependent on RPD1 (and RPD3). Genes regulated by stimuli as diverse as external signals (PH05), cell differentiation processes (SPO11 and SPO13), cell type (RME1, FUS1, H0, TY2, STE6, STE3, and BAR1), and genes whose regulatory signals remain unknown (TRK2) depend on RPD1 to achieve maximal states of transcriptional regulation. RPD1 enhances both positive and negative regulation of these genes: in rpdl{Delta} mutants, higher levels of expression are observed under repression conditions and lower levels are observed under activation conditions. They show that several independent genetic screens, designed to identify yeast transcriptional regulator, have detected the RPD1 locus (also known as SIN3, SD11, and UME4). The inferred RPD1 protein contains four regions predicted to take on helix-loop-helix-like secondary structures and three regions (acidic, glutamine rich, and proline rich) reminiscent of the activating domains of transcriptional activators.

• OSTI ID: 5118078
• Journal Information: Molecular and Cellular Biology; (United States), Vol. 11:12; ISSN 0270-7306
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GENES
DNA SEQUENCING
TRANSCRIPTION
GENE REGULATION
CELL DIFFERENTIATION
GLUTAMINE
PROLINE
SACCHAROMYCES CEREVISIAE
AMIDES
AMINES
AMINO ACIDS
AZOLES
CARBOXYLIC ACIDS
EUMYCOTA
FUNGI
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
MICROORGANISMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PLANTS
PYRROLES
PYRROLIDINES
SACCHAROMYCES
STRUCTURAL CHEMICAL ANALYSIS
YEASTS
550200* - Biochemistry