Laser videofluorometer system for real-time characterization of high-performance liquid chromatographic eluate. [3-hydroxy-benzo(a)pyrene]
A second generation videofluorometer has been developed for real-time characterization of high-performance liquid chromatographic eluate. The instrument features a nitrogen-laser-pumped dye laser as excitation source and quarter meter polychromator/microchannel plate-intensified diode array as fluorescence detector. The dye laser cavity is tuned with a moving-iron galvanometer scanner grating drive, permitting the laser output to be changed to any wavelength in its range in less than 40 ms. Thus, the optimum excitation wavelength can be chosen for each chromatographic region. A minimum detection limit of 13 pptr has been obtained for 3-hydroxy-benzo(a)pyrene in a conventional fluorescence cuvette with a 30-s data acquisition. For the same substance eluted chromatographically, a minimum detection limit of 50 pg has been obtained, and a linear dynamic range of greater than 3 orders of magnitude observed. An extract of soil that had been contaminated with polyaromatic hydrocarbons was analyzed as a practical test of the system, permitting the quantitation of three known species, and the identification and quantitation of a previously unknown fourth compound.
- Research Organization:
- Univ. of Washington, Seattle
- OSTI ID:
- 5109501
- Journal Information:
- Anal. Chem.; (United States), Vol. 58:13
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
PYRENE
FLUOROSCOPY
LIQUID COLUMN CHROMATOGRAPHY
SOILS
QUANTITATIVE CHEMICAL ANALYSIS
DYE LASERS
EXPERIMENTAL DATA
TRACE AMOUNTS
VIDICONS
AROMATICS
BIOMEDICAL RADIOGRAPHY
CAMERA TUBES
CHEMICAL ANALYSIS
CHROMATOGRAPHY
CONDENSED AROMATICS
DATA
DIAGNOSTIC TECHNIQUES
HYDROCARBONS
IMAGE TUBES
INFORMATION
LASERS
LIQUID LASERS
MEDICINE
NUCLEAR MEDICINE
NUMERICAL DATA
ORGANIC COMPOUNDS
RADIOLOGY
SEPARATION PROCESSES
400105* - Separation Procedures
400104 - Spectral Procedures- (-1987)