Reversal of Fv-1 host range by in vitro restriction endonuclease fragment exchange between molecular clones of N-tropic and B-tropic murine leukemia virus genomes

Journal Article · Sat Oct 01 00:00:00 EDT 1983 · J. Virol.; (United States)

OSTI ID:5098936

Boone, L R; Myer, F E; Yang, D M; Ou, C Y; Koh, C K; Roberson, L E; Tennant, R W; Yang, W K

The authors molecularly cloned unintegrated viral DNA of the BALB/c endogenous N-tropic and B-tropic murine leukemia retroviruses and in vitro passage N-tropic Gross (passage A) murine leukemia retroviruses. Recombinant genomes were constructed in vitro by exchanging homologous restriction enzyme fragments from N- or B-tropic parents and subsequent recloning. Infectious virus was recovered after transfection of these recombinant genomes into NIH-3T3 cells and cocultivation with the Fv-1 nonrestrictive SC-1 cells. XC plaque assays of recombinant virus progeny on Fv-1/sup n/ and Fv-1/sup b/ cells indicated that the Fv-1 host range was determined by sequences located between the BamHI site in the p30 region of the gag gene (1.6 kilobase pairs from the left end of the map) and the HindIII site located in pol gene (2.9 kilobase pairs from the left end of the map).

• Research Organization: Oak Ridge National Lab., TN
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 5098936
• Journal Information: J. Virol.; (United States), Vol. 48:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
LEUKEMIA VIRUSES
DNA-CLONING
GENETIC MAPPING
RECOMBINANT DNA
MOLECULAR BIOLOGY
GENES
IN VITRO
MICE
NUCLEASES
ANIMALS
CLONING
DNA
ENZYMES
ESTERASES
HYDROLASES
MAMMALS
MAPPING
MICROORGANISMS
NUCLEIC ACIDS
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PHOSPHODIESTERASES
RODENTS
VERTEBRATES
VIRUSES
550400* - Genetics
550200 - Biochemistry