Betaine aldehyde dehydrogenase isozymes of spinach

Hanson, A D; Weretilnyk, E A; Weigel, P

Title: Betaine aldehyde dehydrogenase isozymes of spinach

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Abstract

Betaine is synthesized in spinach chloroplasts via the pathway Choline ..-->.. Betaine Aldehyde ..-->.. Betaine; the second step is catalyzed by betaine aldehyde dehydrogenase (BADH). The subcellular distribution of BADH was determined in leaf protoplast lysates; BADH isozymes were separated by 6-9% native PAGE. The chloroplast stromal fraction contains a single BADH isozyme (number1) that accounts for > 80% of the total protoplast activity; the extrachloroplastic fraction has a minor isozyme (number2) which migrates more slowly than number1. Both isozymes appear specific for betaine aldehyde, are more active with NAD than NADP, and show a ca. 3-fold activity increase in salinized leaves. The phenotype of a natural variant of isozyme number1 suggests that the enzyme is a dimer.

Authors:: Hanson, A D; Weretilnyk, E A; Weigel, P

Publication Date:: Tue Apr 01 00:00:00 EST 1986

Research Org.:: Michigan State Univ., East Lansing

OSTI Identifier:: 5098189

DOE Contract Number:: AC02-76ER01338

Resource Type:: Conference

Journal Name:: Plant Physiol., Suppl.; (United States)

Additional Journal Information:: Journal Volume: 80:4; Conference: Annual meeting of the American Society of Plant Physiologists, Baton Rouge, LA, USA, 8-12 Jun 1986

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; BETAINE; BIOSYNTHESIS; LEAVES; ENZYME ACTIVITY; BIOLOGICAL PATHWAYS; ENZYMES; NAD; NADP; SPINACH; AMINES; AMINO ACIDS; AMMONIUM COMPOUNDS; CARBOXYLIC ACIDS; COENZYMES; DRUGS; FOOD; LIPOTROPIC FACTORS; NUCLEOTIDES; ORGANIC ACIDS; ORGANIC COMPOUNDS; PLANTS; QUATERNARY COMPOUNDS; SYNTHESIS; VEGETABLES; 550200* - Biochemistry

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                    Hanson, A D, Weretilnyk, E A, and Weigel, P. Betaine aldehyde dehydrogenase isozymes of spinach.  United States: N. p., 1986. 
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                    Hanson, A D, Weretilnyk, E A, & Weigel, P. Betaine aldehyde dehydrogenase isozymes of spinach.  United States.

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                    Hanson, A D, Weretilnyk, E A, and Weigel, P. 1986.  
        "Betaine aldehyde dehydrogenase isozymes of spinach".  United States.

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@article{osti_5098189,

  title        = {Betaine aldehyde dehydrogenase isozymes of spinach},

  author       = {Hanson, A D and Weretilnyk, E A and Weigel, P},

  abstractNote = {Betaine is synthesized in spinach chloroplasts via the pathway Choline ..-->.. Betaine Aldehyde ..-->.. Betaine; the second step is catalyzed by betaine aldehyde dehydrogenase (BADH). The subcellular distribution of BADH was determined in leaf protoplast lysates; BADH isozymes were separated by 6-9% native PAGE. The chloroplast stromal fraction contains a single BADH isozyme (number1) that accounts for > 80% of the total protoplast activity; the extrachloroplastic fraction has a minor isozyme (number2) which migrates more slowly than number1. Both isozymes appear specific for betaine aldehyde, are more active with NAD than NADP, and show a ca. 3-fold activity increase in salinized leaves. The phenotype of a natural variant of isozyme number1 suggests that the enzyme is a dimer.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/5098189},
  journal      = {Plant Physiol., Suppl.; (United States)},
number       = ,

  volume       = 80:4,

  place        = {United States},

  year         = {Tue Apr 01 00:00:00 EST 1986},

  month        = {Tue Apr 01 00:00:00 EST 1986}

}

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