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Title: Detection of an azido-(/sup 14/C)-atrazine labeled protein transferred to nitrocellulose paper

An electrophoretically similar protein in spinach and maize can be covalently labeled with azido-(/sup 14/C)-atrazine and separated by 10-18% gradient LDS-PAGE. The protein profile can be transferred to nitrocellulose paper (ncp) by western blotting. The ncp containing the protein profile is sliced into 2 mm slices and counted with liquid scintillation. The labeled protein migrates as a diffuse band with a Mr of 34 kD. This band migrates at a higher Mr (40 kD) under different gel conditions. The ncp dissolves in the organic scintillation cocktail thus providing a more sensitive and quantitative detection of the /sup 14/C. This technique allows the simultaneous immunological and radiochemical identification of many electrophoretically separable proteins.
Authors:
; ;
Publication Date:
OSTI Identifier:
5098188
Resource Type:
Conference
Resource Relation:
Journal Name: Plant Physiol., Suppl.; (United States); Journal Volume: 80:4; Conference: Annual meeting of the American Society of Plant Physiologists, Baton Rouge, LA, USA, 8-12 Jun 1986; Other Information: C45.00*
Research Org:
Dept. of Biological Sciences, Denver, CO
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; MAIZE; PROTEINS; LABELLING; SPINACH; CARBON 14 COMPOUNDS; ELECTROPHORESIS; NITROCELLULOSE; SEPARATION PROCESSES; CARBOHYDRATES; CELLULOSE ESTERS; CEREALS; CHEMICAL EXPLOSIVES; ESTERS; EXPLOSIVES; FOOD; GRASS; LABELLED COMPOUNDS; NITRIC ACID ESTERS; ORGANIC COMPOUNDS; PLANTS; POLYSACCHARIDES; SACCHARIDES; VEGETABLES 550200* -- Biochemistry