Immunocytochemical localization and subcellular site of lectin synthesis in developing wheat embryos

Conference · Tue Apr 01 00:00:00 EST 1986 · Plant Physiol., Suppl.; (United States)

OSTI ID:5096125

Raikhel, N V; Peumans, W J

Appearance of wheat germ agglutinin (WGA) during wheat germ embryogenesis was studied using indirect immunocytochemical methods at the light and electron microscope levels. Developing embryos were labelled with (/sup 35/S) cysteine in pulse and pulse-chase experiments to study the synthesis and transport of the lectin to protein bodies (PB). The radical, and coleorhiza first accumulated WGA around 10 days post anthesis (DPA), while WGA was found in the epiblast and coleoptile 15 and 20 DPA respectively. At the subcellular level WGA can be seen first in a small developing PB which later fused with larger ones. WGA was distributed evenly throughout the PB. When tissue was pulse-labelled, fractionated on an isopycnic sucrose gradient and exposed to detergent, the incorporated radioactivity of released lectin coincided with the position of the endoplasmic reticulum (ER) marker enzyme NADH-cytochrome c reductase. Both radioactivity and enzyme activity shifted to a higher density in the presence of 2 mM Mg acetate, indicating that radioactive lectin was associated with the rough ER.

• Research Organization: Univ. of Georgia, Athens
• OSTI ID: 5096125
• Journal Information: Plant Physiol., Suppl.; (United States), Vol. 80:4; Conference: Annual meeting of the American Society of Plant Physiologists, Baton Rouge, LA, USA, 8-12 Jun 1986
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
LECTINS
BIOLOGICAL LOCALIZATION
BIOSYNTHESIS
WHEAT
EMBRYOS
ENZYME ACTIVITY
MAGNESIUM COMPOUNDS
PLANT CELLS
RADIONUCLIDE KINETICS
SULFUR 35
TRACER TECHNIQUES
ALKALINE EARTH METAL COMPOUNDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CEREALS
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
GRASS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
NUCLEI
PLANTS
RADIOISOTOPES
SULFUR ISOTOPES
SYNTHESIS
550201* - Biochemistry- Tracer Techniques