Phosphotransferase activity in Clostridium acetobutylicum from acidogenic and solventogenic phases of growth

Journal Article · Thu May 01 00:00:00 EDT 1986 · Appl. Environ. Microbiol.; (United States)

OSTI ID:5089957

Hutkins, R W; Kashket, E R

Clostridium acetobutylicum cells, when energized with fructose, transported and phosphorylated the glucose analog 2-deoxyglucose by a phosphoenolpyruvate-dependent phosphotransferase (PT) system. Butanol up to 2% did not inhibit PT activity, although its chaotropic effect on the cell membrane caused cellular phosphoenolpyruvate and the 2-deoxyglucose-6-phosphate to leak out. Cells harvested from the solventogenic phase of batch growth had a significantly lower PT activity than did cells from the acidogenic phase.

• Research Organization: Boston Univ. School of Medicine, MA
• OSTI ID: 5089957
• Journal Information: Appl. Environ. Microbiol.; (United States), Vol. 51:5
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
BUTANOLS
BIOLOGICAL EFFECTS
CLOSTRIDIUM ACETOBUTYLICUM
ENZYME ACTIVITY
GLUCOSE
METABOLISM
PHOSPHOTRANSFERASES
ALCOHOLS
ALDEHYDES
BACTERIA
CARBOHYDRATES
CLOSTRIDIUM
ENZYMES
HEXOSES
HYDROXY COMPOUNDS
METHANOGENIC BACTERIA
MICROORGANISMS
MONOSACCHARIDES
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
SACCHARIDES
TRANSFERASES
560302* - Chemicals Metabolism & Toxicology- Microorganisms- (-1987)
550700 - Microbiology