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Title: Comparative mutagenicity studies of azo dyes and their reduction products in Salmonella typhimurium

Abstract

The arabinose-resistant and Ames assay systems of Salmonella typhimurium were used to evaluate the mutagenic potential of azo dyes and their aromatic amine reduction products. Azo dyes, namely direct black 38, direct blue 15, and direct red 2, were mutagenic in the arabinose-resistant and Ames assays with both hamster and rat liver S9 activation. Both assays gave relatively higher mutagenic responses with hamster S9. Reduction products of these dyes, namely benzidine, o-dianisidine, and o-tolidine, were mutagenic in the Ames assay. Benzidine was weakly mutagenic and o-dianisidine and o-tolidine were nonmutagenic in the arabinose-resistant assay. These results indicate that both arabinose-resistant tester SV50 and Ames tester TA98 were sensitive in detecting mutagenicity of azo dyes. The use of the standard plate protocol with Ames tester TA98 is more efficient than the modified azo dye protocol in detecting mutagenicity of aromatic amine reduction products. Additional modifications in either the standard plate or modified azo dye protocols may improve detection of mutagenicity of these compounds in the arabinose-resistant assay system.

Authors:
; ;
Publication Date:
Research Org.:
West Virginia Univ., Morgantown
OSTI Identifier:
5089856
Resource Type:
Journal Article
Journal Name:
J. Toxicol. Environ. Health; (United States)
Additional Journal Information:
Journal Volume: 18:1
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; AZO DYES; MUTAGEN SCREENING; MUTAGENESIS; COMPARATIVE EVALUATIONS; EXPERIMENTAL DATA; HAMSTERS; LIVER; METABOLIC ACTIVATION; METABOLITES; RATS; SALMONELLA TYPHIMURIUM; ANIMALS; AZO COMPOUNDS; BACTERIA; BODY; DATA; DIGESTIVE SYSTEM; DYES; GLANDS; INFORMATION; MAMMALS; MICROORGANISMS; NUMERICAL DATA; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; RODENTS; SALMONELLA; SCREENING; VERTEBRATES; 560302* - Chemicals Metabolism & Toxicology- Microorganisms- (-1987)

Citation Formats

Krishna, G, Xu, J, and Nath, J. Comparative mutagenicity studies of azo dyes and their reduction products in Salmonella typhimurium. United States: N. p., 1986. Web. doi:10.1080/15287398609530852.
Krishna, G, Xu, J, & Nath, J. Comparative mutagenicity studies of azo dyes and their reduction products in Salmonella typhimurium. United States. https://doi.org/10.1080/15287398609530852
Krishna, G, Xu, J, and Nath, J. 1986. "Comparative mutagenicity studies of azo dyes and their reduction products in Salmonella typhimurium". United States. https://doi.org/10.1080/15287398609530852.
@article{osti_5089856,
title = {Comparative mutagenicity studies of azo dyes and their reduction products in Salmonella typhimurium},
author = {Krishna, G and Xu, J and Nath, J},
abstractNote = {The arabinose-resistant and Ames assay systems of Salmonella typhimurium were used to evaluate the mutagenic potential of azo dyes and their aromatic amine reduction products. Azo dyes, namely direct black 38, direct blue 15, and direct red 2, were mutagenic in the arabinose-resistant and Ames assays with both hamster and rat liver S9 activation. Both assays gave relatively higher mutagenic responses with hamster S9. Reduction products of these dyes, namely benzidine, o-dianisidine, and o-tolidine, were mutagenic in the Ames assay. Benzidine was weakly mutagenic and o-dianisidine and o-tolidine were nonmutagenic in the arabinose-resistant assay. These results indicate that both arabinose-resistant tester SV50 and Ames tester TA98 were sensitive in detecting mutagenicity of azo dyes. The use of the standard plate protocol with Ames tester TA98 is more efficient than the modified azo dye protocol in detecting mutagenicity of aromatic amine reduction products. Additional modifications in either the standard plate or modified azo dye protocols may improve detection of mutagenicity of these compounds in the arabinose-resistant assay system.},
doi = {10.1080/15287398609530852},
url = {https://www.osti.gov/biblio/5089856}, journal = {J. Toxicol. Environ. Health; (United States)},
number = ,
volume = 18:1,
place = {United States},
year = {Wed Jan 01 00:00:00 EST 1986},
month = {Wed Jan 01 00:00:00 EST 1986}
}