Transposon mutagenesis in Desulfovibrio desulfuricans: Development of a random mutagenesis tool from Tn7
- Univ. of Missouri, Columbia, MO (United States); and others
The transposons Tn5, Tn7, Tn9, and Tn10 or their derivatives have been examined for transposition in the sulfate-reducing bacterium Desulfovibrio desulfuricans G20. Tn7 inserted with a frequency of 10{sup {minus}4} to 10{sup {minus}3} into a unique attachment site that shows strong homology with those sites identified in other gram-negative bacteria. Inactivation of the tnsD gene in Tn7, encoding the function directing insertion into the unique site, yielded a derivative that transposed essentially randomly with a frequency of ca. 10{sup {minus}6} per donor. Derivatives of Tn5, but not wild-type Tn5, were also found to undergo random transposition at a similar frequency. No evidence was obtained for transposition of Tn9 or Tn10. 34 refs., 5 figs., 2 tabs.
- DOE Contract Number:
- FG02-87ER13713
- OSTI ID:
- 508353
- Journal Information:
- Applied and Environmental Microbiology, Vol. 62, Issue 10; Other Information: PBD: Oct 1996
- Country of Publication:
- United States
- Language:
- English
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