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Title: Amphibian alcohol dehydrogenase, the major frog liver enzyme. Relationships to other forms and assessment of an early gene duplication separating vertebrate class I and class III alcohol dehydrogenases

Submammalian alcohol dehydrogenase structures can be used to evaluate the origins and functions of different types of the mammalian enzyme. Two avian forms were recently reported, and the authors now define the major amphibian alcohol dehydrogenase. The enzyme from the liver of the Green frog Rana perezi was purified, carboxymethylated, and submitted to amino acid sequence determination by peptide analysis of six different digest. The protein has a 375-residue subunit and is a class I alcohol dehydrogenase, bridging the gap toward the original separation of the classes that are observable in the human alcohol dehydrogenase system. In relation to the human class I enzyme, the amphibian protein has residue identities exactly halfway (68%) between those for the corresponding avian enzyme (74%) and the human class III enzyme (62%), suggesting an origin of the alcohol dehnydrogenase classes very early in or close to the evolution of the vertebrate line. This conclusion suggests that these enzyme classes are more universal among animals than previously realized and constitutes the first real assessment of the origin of the duplications leading to the alcohol dehydrogenase classes. In conclusion, the amphibian enzyme allows a rough positioning of the divergence of the alcohol dehydrogenase classes, shows thatmore » the class I type is widesprread in vertebrates, and functionally conforms with greater variations at the substrate-binding than the coenzyme-binding site.« less
Authors:
;  [1] ; ;  [2]
  1. (Karolinska Inst., Stockholm (Sweden))
  2. (Univ. Autonoma de Barcelona (Spain))
Publication Date:
OSTI Identifier:
5081503
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemistry; (United States); Journal Volume: 30:11
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ALCOHOL DEHYDROGENASE; GENE REGULATION; ENZYMES; BIOLOGICAL EVOLUTION; AMINO ACID SEQUENCE; DNA SEQUENCING; FROGS; LIQUID COLUMN CHROMATOGRAPHY; LIVER; PURIFICATION; AMPHIBIANS; ANIMALS; AQUATIC ORGANISMS; BODY; CHROMATOGRAPHY; DIGESTIVE SYSTEM; GLANDS; HEMIACETAL DEHYDROGENASES; MOLECULAR STRUCTURE; ORGANS; OXIDOREDUCTASES; SEPARATION PROCESSES; STRUCTURAL CHEMICAL ANALYSIS; VERTEBRATES 550200* -- Biochemistry