Autoradiographic disposition of (1-methyl-/sup 14/C)- and (2-/sup 14/C)caffeine in mice

Journal Article · Tue Nov 01 00:00:00 EST 1983 · Toxicol. Appl. Pharmacol.; (United States)

DOI:https://doi.org/10.1016/0041-008X(83)90340-X· OSTI ID:5077992

Lachance, M P; Marlowe, C; Waddell, W J

Male, C57B1/6J mice received either (1-methyl-14C)caffeine or (2-14C)caffeine via the tail vein at a dose of 0.7 or 11 mg/kg, respectively. At 0.1, 0.33, 1, 3, 9, and 24 hr after treatment, the mice were anesthetized with ether and frozen by immersion in dry ice/hexane. The mice were processed for whole-body autoradiography by the Ullberg technique; this procedure does not allow thawing or contact with solvents. All autoradiographs revealed some retention of radioactivity at early time intervals in the lacrimal glands, seminal vesicle fluid, nasal and olfactory epithelium, and retinal melanocytes. The remaining portion of the animal was densitometrically uniform except for the lower levels noted in the CNS and adipose tissues. Excretion of radioactivity by the liver and kidneys seems to be the major routes of elimination. Localization in the liver at late time intervals was confined principally to the centrilobular region. Late sites of retention, observed only after (1-methyl-14C)caffeine administration, included the pancreas, minor and major salivary glands, splenic red pulp, thymal cortex, bone marrow, and gastrointestinal epithelium. Sites of localization present in both studies included the olfactory epithelium, lacrimal glands, hair follicles, and retinal melanocytes. Further studies are needed to determine whether the localization at these various sites is due to metabolic degradation, active transport, or possibly a specific receptor interaction.

• Research Organization: Univ. of Louisville, KY
• OSTI ID: 5077992
• Journal Information: Toxicol. Appl. Pharmacol.; (United States), Vol. 71:2, Issue 2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CAFFEINE
TISSUE DISTRIBUTION
MICE
AUTORADIOGRAPHY
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL LOCALIZATION
CARBON 14 COMPOUNDS
EXCRETION
LABELLED COMPOUNDS
RETENTION FUNCTIONS
ANALEPTICS
ANIMALS
AROMATICS
AZAARENES
CENTRAL NERVOUS SYSTEM AGENTS
CLEARANCE
DISTRIBUTION
DRUGS
FUNCTIONS
HETEROCYCLIC COMPOUNDS
KINETICS
MAMMALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PURINES
REACTION KINETICS
RODENTS
VERTEBRATES
XANTHINES
550501* - Metabolism- Tracer Techniques