Enzymatic synthesis of C-11 formaldehyde: concise communication
An enzymatic synthesis of C-11 formaldehyde from C-11 methanol is presented, with immobilized alcohol oxidase and catalase: a rapid, simple procedure, with a high and reproducible yield. Carbon-11 methanol is oxidized to C-11 formaldehyde by passage over a column on which the enzymes alcohol oxidase and catalase are immobilized. The catalase increases reaction velocity by recycling the oxygen, and prevents destruction of the alcohol oxidase by eliminating the excess of hydrogen peroxide. The yield of the enzyme-catalyzed oxidation was 80-95%. A specific activity of 400-450 mCi/..mu..mole was obtained at EOB + 20 min. Various immobilization techniques and the optimal reaction conditions of the immobilized enzymes are investigated.
- Research Organization:
- State Univ. of Ghent, Belgium
- OSTI ID:
- 5075037
- Journal Information:
- J. Nucl. Med.; (United States), Vol. 25:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CARBON 11
CHEMICAL REACTION YIELD
SYNTHESIS
FORMALDEHYDE
RADIOPHARMACEUTICALS
CHEMICAL PREPARATION
CATALASE
IMMOBILIZED ENZYMES
OXIDOREDUCTASES
ALDEHYDES
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
CARBON ISOTOPES
CARBOXYLIC ACIDS
DRUGS
ENZYMES
EVEN-ODD NUCLEI
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MINUTES LIVING RADIOISOTOPES
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEROXIDASES
PORPHYRINS
RADIOISOTOPES
YIELDS
400702* - Radiochemistry & Nuclear Chemistry- Properties of Radioactive Materials