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Title: On-column digestion of protein for peptide mapping by capillary zone electrophoresis with laser-induced native fluorescence detection

Abstract

We have developed a novel technique to separate and detect peptide fragments which are digested on the same column. In this procedure, pepsin is used to digest low femtomole amounts of [beta]-lactoglobulin on the column. Then, CZE and LINF are applied to separate and detect the peptide fragments. The advantages of this method are its simplicity, high sensitivity, high selectivity, efficient operation, and high speed. 38 refs., 6 figs., 2 tabs.

Authors:
;  [1]
  1. Ames Lab., IA (United States) Iowa State Univ., Ames, IA (United States)
Publication Date:
OSTI Identifier:
5069899
DOE Contract Number:  
W-7405-ENG-82
Resource Type:
Journal Article
Journal Name:
Analytical Chemistry (Washington); (United States)
Additional Journal Information:
Journal Volume: 65:20; Journal ID: ISSN 0003-2700
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES; GLOBULINS; DIGESTION; PEPTIDES; QUALITATIVE CHEMICAL ANALYSIS; SEPARATION PROCESSES; PROTEINS; DETECTION; CAPILLARY FLOW; COMPILED DATA; ELECTROPHORESIS; FLUORESCENCE; LASER SPECTROSCOPY; PEPSIN; ACID PROTEINASES; CHEMICAL ANALYSIS; DATA; ENZYMES; FLUID FLOW; HYDROLASES; INFORMATION; LUMINESCENCE; NUMERICAL DATA; ORGANIC COMPOUNDS; PEPTIDE HYDROLASES; SPECTROSCOPY; 400100* - Analytical & Separations Chemistry; 550200 - Biochemistry

Citation Formats

Chang, H T, and Yeung, E S. On-column digestion of protein for peptide mapping by capillary zone electrophoresis with laser-induced native fluorescence detection. United States: N. p., 1993. Web. doi:10.1021/ac00068a040.
Chang, H T, & Yeung, E S. On-column digestion of protein for peptide mapping by capillary zone electrophoresis with laser-induced native fluorescence detection. United States. https://doi.org/10.1021/ac00068a040
Chang, H T, and Yeung, E S. 1993. "On-column digestion of protein for peptide mapping by capillary zone electrophoresis with laser-induced native fluorescence detection". United States. https://doi.org/10.1021/ac00068a040.
@article{osti_5069899,
title = {On-column digestion of protein for peptide mapping by capillary zone electrophoresis with laser-induced native fluorescence detection},
author = {Chang, H T and Yeung, E S},
abstractNote = {We have developed a novel technique to separate and detect peptide fragments which are digested on the same column. In this procedure, pepsin is used to digest low femtomole amounts of [beta]-lactoglobulin on the column. Then, CZE and LINF are applied to separate and detect the peptide fragments. The advantages of this method are its simplicity, high sensitivity, high selectivity, efficient operation, and high speed. 38 refs., 6 figs., 2 tabs.},
doi = {10.1021/ac00068a040},
url = {https://www.osti.gov/biblio/5069899}, journal = {Analytical Chemistry (Washington); (United States)},
issn = {0003-2700},
number = ,
volume = 65:20,
place = {United States},
year = {Fri Oct 15 00:00:00 EDT 1993},
month = {Fri Oct 15 00:00:00 EDT 1993}
}