(Hydroxyproline-rich glycoprotein of the plant cell wall): Report on work from June 1987 to June 1988

Technical Report · Fri Jan 01 00:00:00 EST 1988

OSTI ID:5068518

In soybean seed costs the accumulation of the hydroxproline-rich glycoprotein extensin is regulated in a developmental and tissue-specific manner. The time course of appearance of extensin during seed development was studied by Western blot analysis and by immunogold-silver localization. Using these techniques extensin was first detected at 16 to 18 d after anthesis, increasing during development to high levels at 24 d after anthesis. Immunogold-silver localization of extensin in the seed coat showed marked depostion of the glycoprotein in the walls of palisade epidermal cells and hourglass cells. The immunolocalization of extensin in developing soybean seeds was also made by a new technique - tissue printing on nitrocellulose paper. This technique shows that extensin is primarily localized in the seed coal, hilum, and vascular elements of the seed.

• Research Organization: Washington Univ., St. Louis, MO (USA)
• DOE Contract Number: FG02-84ER13255
• OSTI ID: 5068518
• Report Number(s): DOE/ER/13255-1; ON: DE88010534
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
GLUCOPROTEINS
IMMUNOASSAY
AMINO ACIDS
CARROTS
ELECTROPHORESIS
GLYCINE HISPIDA
PROGRESS REPORT
SUBCELLULAR DISTRIBUTION
BACTERIA
CARBOHYDRATES
CARBOXYLIC ACIDS
DISTRIBUTION
DOCUMENT TYPES
FOOD
LEGUMINOSAE
MICROORGANISMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLANTS
PROTEINS
RHIZOBIUM
SACCHARIDES
VEGETABLES
140504* - Solar Energy Conversion- Biomass Production & Conversion- (-1989)
550200 - Biochemistry
553000 - Agriculture & Food Technology