Comparative study on the mechanisms of rotavirus inactivation by sodium dodecyl sulfate and ethylenediaminetetraacetate
- Sandia Labs., Albuquerque, NM
This report describes a comparative study on the effects of the anionic detergent sodium dodecyl sulfate and the chelating agent ethylenediaminetetraacetate on purified rotavirus SA-11 particles. Both chemicals readily inactivated rotavirus at quite low concentrations and under very mild conditions. In addition, both agents modified the viral capsid and prevented the adsorption of inactivated virions to cells. Capsid damage by ethylenediaminetetraacetate caused a shift in the densities of rotavirions from about l.35 to about 1.37 g/ml and a reduction in their sedimentation coefficients. Sodium dodcyl sulfate, on the other hand, did not detectably alter either of these physical properties of rotavirions. Both agents caused some alteration of the isoelectric points of the virions. Finally, analysis of rotavirus proteins showed that ethylenediaminetetraacetate caused the loss of two protein peaks from the electrophoretic pattern of virions but sodium dodecyl sulfate caused the loss of only one of these same protein peaks.
- DOE Contract Number:
- E(29-2)-3536; EPA-IAG-D6-0675
- OSTI ID:
- 5059786
- Journal Information:
- Appl. Environ. Microbiol.; (United States), Vol. 39:6
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
EDTA
BIOLOGICAL EFFECTS
SIMIAN VIRUS
INACTIVATION
SODIUM SULFATES
CARBON 14
COMPARATIVE EVALUATIONS
DETERGENTS
LABELLED COMPOUNDS
PROTEINS
TRITIUM
ULTRASTRUCTURAL CHANGES
URIDINE
ADDITIVES
ALKALI METAL COMPOUNDS
AMINO ACIDS
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBON ISOTOPES
CARBOXYLIC ACIDS
CHELATING AGENTS
EMULSIFIERS
EVEN-EVEN NUCLEI
HETEROCYCLIC COMPOUNDS
HYDROGEN ISOTOPES
HYDROXY COMPOUNDS
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
MORPHOLOGICAL CHANGES
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PARASITES
PYRIMIDINES
RADIOISOTOPES
RIBOSIDES
SODIUM COMPOUNDS
SULFATES
SULFUR COMPOUNDS
SURFACTANTS
URACILS
VIRUSES
WETTING AGENTS
YEARS LIVING RADIOISOTOPES
550701* - Microbiology- Tracer Techniques
560302 - Chemicals Metabolism & Toxicology- Microorganisms- (-1987)
550201 - Biochemistry- Tracer Techniques