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Title: Specific estrogen-induced cell proliferation of cultured Syrian hamster renal proximal tubular cells in serum-free chemically defined media

Abstract

It has long been recognized that the renal proximal tubular epithelium of the hamster is a bona fide estrogen target tissue. The effect of estrogens on the growth of proximal tubule cell explants and dissociated single cells derived from these explant outgrowths has been studied in culture. Renal tubular cells were grown on a PF-HR-9 basement membrane under serum-free chemically defined culture conditions. At 7-14 days in culture, cell number was enhanced 3-fold in the presence of either 17{beta}-estradiol or diethylstilbestrol. A similar 3-fold increase in cell number was also seen at 1 nM 17{beta}-estradiol in subcultured dissociated single tubular cells derived from hamster renal tubular explant outgrowths at 21 days in culture. Concomitant exposure of tamoxifen at 3-fold molar excess in culture completely abolished the increase in cell number seen with 17{beta}-estradiol. The proliferation effect of estrogens on proximal tubular cell growth appears to be species specific since 17{beta}-estradiol did not alter the growth of either rat or guinea pig proximal tubules in culture. In addition, at 7-10 days in culture in the presence of 17{beta}-estradiol, ({sup 3}H)thymidine labeling of hamster tubular cells was enhanced 3-fold. These results clearly indicate that estrogens can directly induce primary epithelial cell proliferationmore » at physiologic concentrations and provide strong additional evidence for an important hormonal role in the neoplastic transformation of the hamster kidney.« less

Authors:
; ; ; ;  [1]; ;  [2]
  1. Univ. of Wisconsin Medical School, Madison (USA)
  2. Univ. of Minnesota Medical School, Minneapolis (USA)
Publication Date:
OSTI Identifier:
5052297
Resource Type:
Journal Article
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America; (USA)
Additional Journal Information:
Journal Volume: 86:6; Journal ID: ISSN 0027-8424
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ANIMAL CELLS; CELL PROLIFERATION; ESTROGENS; BIOLOGICAL EFFECTS; STEROID HORMONES; CARCINOGENESIS; CELL CULTURES; CYTOLOGICAL TECHNIQUES; GUINEA PIGS; HAMSTERS; KIDNEYS; ONCOGENIC TRANSFORMATIONS; RATS; THYMIDINE; TRITIUM COMPOUNDS; ANIMALS; AZINES; BODY; CELL TRANSFORMATIONS; HETEROCYCLIC COMPOUNDS; HORMONES; HYDROGEN COMPOUNDS; MAMMALS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PATHOGENESIS; PYRIMIDINES; RIBOSIDES; RODENTS; VERTEBRATES; 550301* - Cytology- Tracer Techniques

Citation Formats

Oberley, T D, Lauchner, L J, Pugh, T D, Gonzalez, A, Goldfarb, S, Li, S A, and Li, J J. Specific estrogen-induced cell proliferation of cultured Syrian hamster renal proximal tubular cells in serum-free chemically defined media. United States: N. p., 1989. Web. doi:10.1073/pnas.86.6.2107.
Oberley, T D, Lauchner, L J, Pugh, T D, Gonzalez, A, Goldfarb, S, Li, S A, & Li, J J. Specific estrogen-induced cell proliferation of cultured Syrian hamster renal proximal tubular cells in serum-free chemically defined media. United States. https://doi.org/10.1073/pnas.86.6.2107
Oberley, T D, Lauchner, L J, Pugh, T D, Gonzalez, A, Goldfarb, S, Li, S A, and Li, J J. 1989. "Specific estrogen-induced cell proliferation of cultured Syrian hamster renal proximal tubular cells in serum-free chemically defined media". United States. https://doi.org/10.1073/pnas.86.6.2107.
@article{osti_5052297,
title = {Specific estrogen-induced cell proliferation of cultured Syrian hamster renal proximal tubular cells in serum-free chemically defined media},
author = {Oberley, T D and Lauchner, L J and Pugh, T D and Gonzalez, A and Goldfarb, S and Li, S A and Li, J J},
abstractNote = {It has long been recognized that the renal proximal tubular epithelium of the hamster is a bona fide estrogen target tissue. The effect of estrogens on the growth of proximal tubule cell explants and dissociated single cells derived from these explant outgrowths has been studied in culture. Renal tubular cells were grown on a PF-HR-9 basement membrane under serum-free chemically defined culture conditions. At 7-14 days in culture, cell number was enhanced 3-fold in the presence of either 17{beta}-estradiol or diethylstilbestrol. A similar 3-fold increase in cell number was also seen at 1 nM 17{beta}-estradiol in subcultured dissociated single tubular cells derived from hamster renal tubular explant outgrowths at 21 days in culture. Concomitant exposure of tamoxifen at 3-fold molar excess in culture completely abolished the increase in cell number seen with 17{beta}-estradiol. The proliferation effect of estrogens on proximal tubular cell growth appears to be species specific since 17{beta}-estradiol did not alter the growth of either rat or guinea pig proximal tubules in culture. In addition, at 7-10 days in culture in the presence of 17{beta}-estradiol, ({sup 3}H)thymidine labeling of hamster tubular cells was enhanced 3-fold. These results clearly indicate that estrogens can directly induce primary epithelial cell proliferation at physiologic concentrations and provide strong additional evidence for an important hormonal role in the neoplastic transformation of the hamster kidney.},
doi = {10.1073/pnas.86.6.2107},
url = {https://www.osti.gov/biblio/5052297}, journal = {Proceedings of the National Academy of Sciences of the United States of America; (USA)},
issn = {0027-8424},
number = ,
volume = 86:6,
place = {United States},
year = {Wed Mar 01 00:00:00 EST 1989},
month = {Wed Mar 01 00:00:00 EST 1989}
}