Primary structure of keratinocyte transglutaminase

Journal Article · Sat Dec 01 00:00:00 EST 1990 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.87.23.9333· OSTI ID:5044523

Phillips, M A; Stewart, B E; Qin, Q; Rice, R H ^[1]; Chakravarty, R ^[2]; Floyd, E E; Jetten, A M ^[3]

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1. Univ. of California, Davis (United States)
2. Univ. of Cincinnati Medical Center, OH (United States)
3. National Inst. of Environmental Health Sciences, Research Triangle Park, NC (United States)

The nucleotide and deduced amino acid sequences of the coding regions of human and rat keratinocyte transglutaminases (protein-glutamine: amine {gamma}-glutamyltransferase; EC 2.3.2.13) have been determined. These yield proteins of {approximately}90 kDa that are 92% identical, indicative of the conservation of important structural features. Alignments of amino acid sequences show substantial similarity among the keratinocyte transglutaminase, human clotting factor XIII catalytic subunit, guinea pig liver tissue transglutaminase, and the human erythrocyte band-4.2 protein. The keratinocyte enzyme is most similar to factor XIII, whereas the band-4.2 protein is most similar to the tissue transglutaminase. A salient feature of the keratinocyte transglutaminase is its 105-residue extension beyond the N terminus of the tissue transglutaminase. This extension and the unreltaed activation peptide of factor XIII (a 37-residue extension) appear to be added for specialized functions after divergence of the tissue transglutaminase from their common lineage.

• OSTI ID: 5044523
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 87:23; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
NITROGEN TRANSFERASES
AMINO ACID SEQUENCE
RECOMBINANT DNA
DNA SEQUENCING
BLOOD COAGULATION FACTORS
EPIDERMIS
ERYTHROCYTES
LIVER
MAN
PHOSPHORUS 32
RATS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
COAGULANTS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DNA
DRUGS
ENZYMES
EPITHELIUM
GLANDS
HEMATOLOGIC AGENTS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MATERIALS
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RADIOISOTOPES
RODENTS
SKIN
STRUCTURAL CHEMICAL ANALYSIS
TISSUES
TRANSFERASES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques