Electron spin echo modulation studies of doxylstearic acid spin probes in frozen vesicles: Interaction of the spin probe with D sub 2 O and effects of cholesterol addition
Abstract
Electron spin echo studies have been carried out for a series of x-doxylstearic acid (x = 5, 7, 10, 12 and 16) spin probes in frozen deuteriated aqueous solutions of phospholipid vesicles and cationic dioctadecyldimethylammonium chloride (DODAC) vesicles. Modulation effects due to interactions of the nitroxide group of the spin probes with D{sub 2}O give information about the conformations of the probes and the degree of hydration of the surfactant headgroups as well as about the degree of packing of the alkyl chain. We show that DODAC headgroups are more hydrated than choline headgroups and that the doxylstearic acid probes show a larger tendency for bending in DODAC vesicles than in phospholipid vesicles. Upon addition of cholesterol into phospholipid vesicles, the headgroups are separated and their degree of hydration increases.
- Authors:
-
- Univ. of Houston, TX (USA)
- Publication Date:
- OSTI Identifier:
- 5040244
- Resource Type:
- Journal Article
- Journal Name:
- Journal of Physical Chemistry; (USA)
- Additional Journal Information:
- Journal Volume: 93:4; Journal ID: ISSN 0022-3654
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; CHOLESTEROL; ACOUSTIC ESR; HEAVY WATER; OCTADECANOIC ACID; PHOSPHOLIPIDS; AQUEOUS SOLUTIONS; EXPERIMENTAL DATA; MICELLAR SYSTEMS; CARBOXYLIC ACIDS; DATA; DISPERSIONS; ELECTRON SPIN RESONANCE; ESTERS; HYDROGEN COMPOUNDS; HYDROXY COMPOUNDS; INFORMATION; LIPIDS; MAGNETIC RESONANCE; MIXTURES; MONOCARBOXYLIC ACIDS; NUMERICAL DATA; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC PHOSPHORUS COMPOUNDS; OXYGEN COMPOUNDS; RESONANCE; SOLUTIONS; STEROIDS; STEROLS; WATER; 400201* - Chemical & Physicochemical Properties
Citation Formats
Hiff, T, and Kevan, L. Electron spin echo modulation studies of doxylstearic acid spin probes in frozen vesicles: Interaction of the spin probe with D sub 2 O and effects of cholesterol addition. United States: N. p., 1989.
Web. doi:10.1021/j100341a075.
Hiff, T, & Kevan, L. Electron spin echo modulation studies of doxylstearic acid spin probes in frozen vesicles: Interaction of the spin probe with D sub 2 O and effects of cholesterol addition. United States. https://doi.org/10.1021/j100341a075
Hiff, T, and Kevan, L. 1989.
"Electron spin echo modulation studies of doxylstearic acid spin probes in frozen vesicles: Interaction of the spin probe with D sub 2 O and effects of cholesterol addition". United States. https://doi.org/10.1021/j100341a075.
@article{osti_5040244,
title = {Electron spin echo modulation studies of doxylstearic acid spin probes in frozen vesicles: Interaction of the spin probe with D sub 2 O and effects of cholesterol addition},
author = {Hiff, T and Kevan, L},
abstractNote = {Electron spin echo studies have been carried out for a series of x-doxylstearic acid (x = 5, 7, 10, 12 and 16) spin probes in frozen deuteriated aqueous solutions of phospholipid vesicles and cationic dioctadecyldimethylammonium chloride (DODAC) vesicles. Modulation effects due to interactions of the nitroxide group of the spin probes with D{sub 2}O give information about the conformations of the probes and the degree of hydration of the surfactant headgroups as well as about the degree of packing of the alkyl chain. We show that DODAC headgroups are more hydrated than choline headgroups and that the doxylstearic acid probes show a larger tendency for bending in DODAC vesicles than in phospholipid vesicles. Upon addition of cholesterol into phospholipid vesicles, the headgroups are separated and their degree of hydration increases.},
doi = {10.1021/j100341a075},
url = {https://www.osti.gov/biblio/5040244},
journal = {Journal of Physical Chemistry; (USA)},
issn = {0022-3654},
number = ,
volume = 93:4,
place = {United States},
year = {Thu Feb 23 00:00:00 EST 1989},
month = {Thu Feb 23 00:00:00 EST 1989}
}