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Title: Exogenous gangliosides may affect methylation mechanisms in neuronal cell cultures

Primary neurons in culture from chick embryo cerebral hemispheres were treated with a mixture of gangliosides added to the growth medium (final concentration: 10(-5)M and 10(-8)M) from the 3rd to the 6th day in vitro. Under these conditions methylation processes measured with (3H) and (35S) methionine and (3H)ethanolamine as precursors showed an increased methylation of (3H)ethanolamine containing phospholipids, a correspondent increased conversion of these compounds to (3H)choline containing phospholipids, and a general increased methylation of trichloroacetic acid precipitable macromolecules containing labeled methionine. A small increase in protein synthesis was observed after incubation of neurons with (3H)- and (35S)methionine. This was confirmed after electrophoretic separation of a protein extract with increased 3H- and 35S-labeling in protein bands with moecular weights between 50 and 60 KDaltons. A protein band of about 55 KDaltons appeared to be preferentially labelled when (3H) methionine was the precursor. The treatment with gangliosides increased the incorporation of (methyl-3H) label after incubation of neurons with (3H) methionine, into total DNA and decreased that of total RNA. The treatment of neurons in culture with exogenous gangliosides hence affects differently methylation processes, a finding which may confirm the involvement of gangliosides on the intracellular mediation of neuronal information mechanisms.
Authors:
; ; ;  [1]
  1. (Centre de Neurochimie du CNRS, Strasbourg (France))
Publication Date:
OSTI Identifier:
5031766
Resource Type:
Journal Article
Resource Relation:
Journal Name: Neurochemical Research; (United States); Journal Volume: 16:2
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; GANGLIOSIDES; BIOCHEMICAL REACTION KINETICS; NERVE CELLS; BIOCHEMISTRY; PHOSPHOLIPIDS; METHYLATION; BRAIN; CELL CULTURES; CHICKENS; CHOLINE; DNA; EMBRYOS; METHIONINE; MOLECULAR WEIGHT; RNA; SULFUR 35; TRACER TECHNIQUES; TRITIUM COMPOUNDS; ALCOHOLS; AMINES; AMINO ACIDS; AMMONIUM COMPOUNDS; ANIMAL CELLS; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIRDS; BODY; CARBOHYDRATES; CARBOXYLIC ACIDS; CENTRAL NERVOUS SYSTEM; CHEMICAL REACTIONS; CHEMISTRY; DAYS LIVING RADIOISOTOPES; DRUGS; ESTERS; EVEN-ODD NUCLEI; FOWL; GLYCOLIPIDS; HYDROGEN COMPOUNDS; HYDROXY COMPOUNDS; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; LIGHT NUCLEI; LIPIDS; LIPOTROPIC FACTORS; NERVOUS SYSTEM; NUCLEI; NUCLEIC ACIDS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC PHOSPHORUS COMPOUNDS; ORGANIC SULFUR COMPOUNDS; ORGANS; QUATERNARY COMPOUNDS; RADIOISOTOPES; REACTION KINETICS; SACCHARIDES; SOMATIC CELLS; SULFUR ISOTOPES; VERTEBRATES 550201* -- Biochemistry-- Tracer Techniques