Exogenous gangliosides may affect methylation mechanisms in neuronal cell cultures
- Centre de Neurochimie du CNRS, Strasbourg (France)
Primary neurons in culture from chick embryo cerebral hemispheres were treated with a mixture of gangliosides added to the growth medium (final concentration: 10(-5)M and 10(-8)M) from the 3rd to the 6th day in vitro. Under these conditions methylation processes measured with (3H) and (35S) methionine and (3H)ethanolamine as precursors showed an increased methylation of (3H)ethanolamine containing phospholipids, a correspondent increased conversion of these compounds to (3H)choline containing phospholipids, and a general increased methylation of trichloroacetic acid precipitable macromolecules containing labeled methionine. A small increase in protein synthesis was observed after incubation of neurons with (3H)- and (35S)methionine. This was confirmed after electrophoretic separation of a protein extract with increased 3H- and 35S-labeling in protein bands with moecular weights between 50 and 60 KDaltons. A protein band of about 55 KDaltons appeared to be preferentially labelled when (3H) methionine was the precursor. The treatment with gangliosides increased the incorporation of (methyl-3H) label after incubation of neurons with (3H) methionine, into total DNA and decreased that of total RNA. The treatment of neurons in culture with exogenous gangliosides hence affects differently methylation processes, a finding which may confirm the involvement of gangliosides on the intracellular mediation of neuronal information mechanisms.
- OSTI ID:
- 5031766
- Journal Information:
- Neurochemical Research; (United States), Vol. 16:2; ISSN 0364-3190
- Country of Publication:
- United States
- Language:
- English
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GANGLIOSIDES
BIOCHEMICAL REACTION KINETICS
NERVE CELLS
BIOCHEMISTRY
PHOSPHOLIPIDS
METHYLATION
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CELL CULTURES
CHICKENS
CHOLINE
DNA
EMBRYOS
METHIONINE
MOLECULAR WEIGHT
RNA
SULFUR 35
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALCOHOLS
AMINES
AMINO ACIDS
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ANIMAL CELLS
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BETA-MINUS DECAY RADIOISOTOPES
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CENTRAL NERVOUS SYSTEM
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DAYS LIVING RADIOISOTOPES
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EVEN-ODD NUCLEI
FOWL
GLYCOLIPIDS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
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ORGANIC PHOSPHORUS COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
QUATERNARY COMPOUNDS
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SOMATIC CELLS
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550201* - Biochemistry- Tracer Techniques